内支气管超声波引导的跨支气管针吸收和下一代测序产生了收益
Kristin N Sheehan1, Lara M Khoury2, Angela G Niehaus3
1Department of Pulmonary/Critical Care, Wake Forest University School of Medicine, Winston-Salem, NC, 27157, USA. Knsheeha@wakehealth.edu.
Lung
|April 30, 2024
概括
坚持采用特定的内支气管超声波 (EBUS) 采样协议,几乎使下一代测序 (NGS) 在非小细胞肺癌 (NSCLC) 诊断中的成功率翻了一番.
科学领域:
- 肺部病理学 肺部病理学
- 在瘤学瘤学.
- 病理学 病理学 病理学
背景情况:
- 胸腔内膜超声波 (EBUS) 是肺癌的标准诊断工具.
- 下一代测序 (NGS) 对于识别晚期非状非小细胞肺癌 (NSCLC) 的遗传改变至关重要.
- 目前用于NGS的EBUS协议产生了可变的结果,需要优化.
研究的目的:
- 评估EBUS样本对NGS的收益率,使用已建立的机构协议.
- 确定影响NGS测试样本适度的因素.
- 为开发改进的EBUS分子测试采集协议提供信息.
主要方法:
- 对2016年至2021年期间进行的EBUS支气管检查进行了回顾性审查,用于非状NSCLC诊断.
- 实施标准化采样协议,包括用于免疫组织化学,分子标记物和NGS的冲洗.
- 病理学部门对NGS的样本充分性的评估.
主要成果:
- 分析了278个非状NSCLC样本.
- 对NGS测试的总体样本充分率为57.5%.
- 遵守专门的NGS采样协议显著提高了充分性率 (66.0%与37.2%相比,p<0.001).
结论:
- 与标准程序相比,NGS的一项涉及三到五次专用针冲洗的协议几乎使样品的充分性翻了一番.
- 根据病变大小或位置,没有发现足够性的显著差异.
- 需要进一步的研究来确定最佳的收集和处理方法,以最大限度地保护遗传测序的组织样本.
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