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双重压力因子适应性进化,用于高EPA产量在斯基索基 sp. 和代谢学机制分析

Ying Ou¹, Yu Qin¹, Shoushuai Feng¹

¹The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, WuXi, 214122, People's Republic of China.

Bioprocess and biosystems engineering

|April 30, 2024

概括

适应性进化策略增强了精神分裂症中eicosapentaenoic acid (EPA) 的产生. 这种方法显著提高了EPA产量和生物质,为omega-3脂肪酸生物合成提供了新的途径.

科学领域:

生物技术是生物技术.
微生物发酵微生物发酵
生物化学生物化学

背景情况:

乙酸 (EPA) 是一种具有关键生理作用的必需欧米茄-3多不和脂肪酸 (PUFA).
Schizochytrium是一种微藻类,因其在EPA生产中的潜力而受到认可.

研究的目的:

开发一种适应性的进化策略,以增强精神分裂症中EPA的产生.
研究高温和高氧压力对EPA生物合成和细胞生长的影响.

主要方法:

采用双因素压力策略 (高温和高氧) 适应性演变的精神分裂症.
使用液体色谱-质谱法 (LC-MS) 进行代谢分析.
进行料批发发酵,以评估增强的EPA生产.

主要成果:

与原始菌株相比,进化菌株 (ALE-D50) 的生物质增加了43.85%,EPA产量增加了71.11%.
进化的菌株表现出增强的抗氧化活性,由较低的活性氧物种水平表明.
料批发发酵进一步提高了脂质和EPA的产量,分别提高了54.30%和90.86%.

结论:

在双重压力条件下的适应性进化是增强schizochytrium中EPA生物合成的有效策略.
代谢分析表明,代谢重编程,包括增强的葡萄糖分解,有助于增加EPA的产量.
这项研究为可扩展和高效的EPA生产提供了一个有希望的方法.

关键词:

精神分裂 (Schizochytrium sp.) 是一种疾病.适应性实验室进化的演变.埃可萨丁烯酸是什么埃可萨丁烯酸是什么代谢学代谢学代谢学

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