相关实验视频
Updated: Jun 27, 2025

09:49
Kupffer Cell Isolation for Nanoparticle Toxicity Testing
Published on: August 18, 2015
23.8K
库普弗细胞多样性维持了与酒精相关的肝病的肝功能
Kyo Sasaki1, Sheetalnath Rooge1, Sumedha Gunewardena2
1Department of Internal Medicine, University of Kansas Medical Center, Kansas City, Kansas, USA.
Hepatology (Baltimore, Md.)
|April 30, 2024
概括
肝脏巨细胞是酒精相关肝病 (ALD) 的关键. 库弗弗细胞 (KCs) 是抗炎的,而透的巨细胞促进炎症和纤维化,这是ALD治疗的关键发现.
科学领域:
- 免疫学 免疫学 免疫学
- 肝病学 肝病学是一种肝病学.
- 细胞生物学 细胞生物学
背景情况:
- 肝脏巨细胞,特别是库弗弗细胞 (KC) 和透单细胞,在与酒精有关的肝病 (ALD) 中至关重要.
- 对于各种巨细胞子集在ALD病变发生过程中的独特功能,人们的理解有限.
- 肝脏巨细胞的异质性需要详细研究它们在疾病进展中的作用.
研究的目的:
- 研究肝脏髓状细胞的多样性和功能作用,特别是巨细胞子集,在ALD的西方饮食酒精 (WDA) 鼠标模型中.
- 阐明库普费尔细胞与透单细胞/巨细胞对ALD肝损伤和纤维化的特定贡献.
- 在巨细胞种群中确定潜在的治疗点,用于治疗ALD中的肝衰竭和纤维化.
主要方法:
- 利用单细胞RNA测序,在ALD的WDA小鼠模型中分析肝脏髓状细胞区.
- 在暴露于酒精的过程中,采用了基于喉毒素的系统进行向的Kupffer细胞 (KC) 除.
- 分析了肝脏组织,并进行了整个肝脏RNA测序,以评估细胞变化和基因表达模式.
主要成果:
- 在WDA肝脏中,Kupffer细胞 (KCs) 和透单细胞/巨细胞分别占髓状细胞群约50%.
- 大多数KC子组表现出抗炎和脂质代谢基因特征,其中一个小子组显示出促炎标志物.
- 透的单细胞/巨细胞群主要是亲炎性和亲纤维性;KC切除导致肝功能衰竭和改变肝细胞基因表达,反映了人类酒精性肝炎.
- 基因细胞切除导致肝衰竭,其特征是PT/INR和胆红素的升高,肝细胞分化标记物的丧失,以及原始细胞标记物的增加.
结论:
- 在这种ALD模型中,Kupffer细胞 (KC) 具有抗炎性质,对于维持肝细胞分化至关重要.
- 透的单细胞/巨细胞主要是前炎性,并对肝纤维化发展作出重大贡献.
- 准特定的巨细胞子组为管理ALD肝功能衰竭和纤维化提供了有前途的治疗策略.
相关概念视频
Liver Regeneration
3.3K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.3K
Liver Physiology
518
The liver, an essential organ in the human body, performs over 200 vital functions that can be broadly categorized into metabolic, hematological, endocrine regulation, and bile production.
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of 70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can...
Metabolic Regulation:
The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of 70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can...
518
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Tissue Renewal without Stem Cells
1.7K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
1.7K
Liver Histology
747
The microscopic anatomy of the liver is a complex and intricate system that comprises numerous structural units known as liver lobules, each of which is comparable in size to a sesame seed. These hexagonal structures consist of plates of liver cells or hepatocytes, which are characterized by their versatility and abundance of cellular apparatus like rough and smooth ER, Golgi apparatus, peroxisomes, and mitochondria.
Hepatocytes perform a variety of essential functions. They secrete...
Hepatocytes perform a variety of essential functions. They secrete...
747
Diseases of the Liver and Gallbladder
531
Liver and gallbladder diseases are a significant health concern, with prominent conditions including cirrhosis, hepatitis, non-alcoholic fatty liver disease (NAFLD), and gallstones. Jaundice is a common manifestation of liver and biliary disease.
Cirrhosis is characterized by the scarring of hepatic lobules in the liver, which are replaced by fibrous tissue, affecting the liver's normal functioning. NAFLD, on the other hand, is caused by an excessive build-up of fat in the liver, not...
Cirrhosis is characterized by the scarring of hepatic lobules in the liver, which are replaced by fibrous tissue, affecting the liver's normal functioning. NAFLD, on the other hand, is caused by an excessive build-up of fat in the liver, not...
531

