在生物成像中,微RNA与明亮的光RNA Aptamer通过目标介导的透驱动的触摸交换进行微RNA成像
Yu Gu1, Rui Bai1, Xingchen Qiu2
1Institute of Materials Science and Devices, School of Materials Science and Engineering, Suzhou University of Science and Technology, Kerui Road, Suzhou 215009, P. R. China.
Analytical chemistry
|April 30, 2024
概括
这项研究引入了一种用于在活细胞和动物中成像微RNA (miRNA) 的新方法. 以为驱动的托霍尔德交换 (EDTE) 策略显著提高了敏感和选择性miRNA检测的信号亮度.
科学领域:
- 生物技术是生物技术.
- 分子生物学分子生物学
- 生物成像是一种生物成像.
背景情况:
- 微RNA (miRNA) 在生物过程中至关重要,但由于稀少和光工具有限,在体内成像具有挑战性.
- 现有的RNA体具有较高的亲和力,但其亮度较低,阻碍了有效的生物成像.
研究的目的:
- 开发一种新有效的战略,用于微RNAs的敏感和选择性体内成像.
- 为了克服当前miRNA检测方法中低丰度和低亮度的局限性.
主要方法:
- 采用了基于目标介导驱动托霍尔德交换 (EDTE) 的理性设计.
- 在EDTE策略诱导RNA体释放激活一个光体的miRNA成像.
- 定制识别单元使特定miRNA (例如miRNA-125b,miRNA-21) 的敏感成像成为可能.
主要成果:
- 该EDTE策略实现了选择性miRNA成像,在活细胞和瘤携带小鼠中具有良好的稳定性.
- 证明了不同miRNAs的通用生物成像应用.
- 与细胞内miRNA成像的传统方法相比,信号/噪声比提高了4.6倍.
- 启用了敏感的体内小RNA成像.
结论:
- 该EDTE战略为敏感和选择性的miRNA生物成像 in vitro和 in vivo提供了一个有前途的工具.
- 这种方法增强了与疾病相关的miRNA功能和相互作用的调查.
- 普遍适用性和改进的信号噪声比强调了其在生物医学研究中的潜力.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Nucleic Acid Structure
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
DNA Structure
DNA has a double-helix structure. The...


