七个子组织中的Cis-eQTLs识别了用于生长和尸体特征的新型候选基因
Wentao Cai1, Jian Hu1, Yunsheng Zhang1
1Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
BMC genomics
|April 30, 2024
概括
这项研究绘制了子的表达量特征位点 (eQTLs),识别了影响生长和尸体特征的关键基因. 这些发现为通过遗传洞察改善子生产提供了宝贵的资源.
科学领域:
- 基因组学就是基因组学.
- 动物遗传学动物遗传学
- 分子生物学分子生物学
背景情况:
- 表达量的特征位点 (eQTL) 研究调查了基因变异对基因表达的影响.
- eQTL映射和GWAS的局部化有助于识别复杂特征的基因和变异.
- eQTL映射以前没有在身上进行过,这限制了对它们的经济特征的理解.
研究的目的:
- 在子中进行第一个eQTL分析.
- 识别与经济特征相关的eQTL,特别是生长和尸体质量.
- 为了探索子的组织特异性基因表达调节.
主要方法:
- 利用公开可用的RNA测序数据,来自820个子样本的7种组织 (肝脏,肌肉,血液,脂肪,卵巢,脏,肺).
- 进行了cis-eQTL分析,以确定影响近距离基因表达的遗传变异.
- 对50种子特征的现有全基因组关联研究 (GWAS) 数据进行了局部识别的cis-eQTL.
主要成果:
- 对12,266个基因确定了113,374个cis-eQTL,其中39.1%在多种组织中发现.
- 在整个组织中观察到血液cis-eQTLs的保存率较低,但与肝脏组织的强烈共享模式.
- 发现了基因表达和影响生长和尸体特征的位置之间的新兴关联,确定了像SRSF4,GSS,IGF2BP1,NDUFC2,ELF3和RUNDC1.1这样的候选基因.
结论:
- 在子的基因表达的遗传调节中显示出显著的组织特异性差异.
- 提供了对可能影响子生长和尸体特征的候选基因的见解.
- 建立了一个基础的eQTL数据集,用于未来的基因研究,旨在增强子生产特征.
相关概念视频
Transgenic Organisms
Overview
X-linked Traits
In most mammalian species, females have two X sex chromosomes and males have an X and Y. As a result, mutations on the X chromosome in females may be masked by the presence of a normal allele on the second X. In contrast, a mutation on the X chromosome in males more often causes observable biological defects, as there is no normal X to compensate. Trait variations arising from mutations on the X chromosome are called “X-linked”.
Cis-regulatory Sequences
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
X-linked Traits
In most mammalian species, females have two X sex chromosomes and males have an X and Y. As a result, mutations on the X chromosome in females may be masked by the presence of a normal allele on the second X. In contrast, a mutation on the X chromosome in males more often causes observable biological defects, as there is no normal X to compensate. Trait variations arising from mutations on the X chromosome are called “X-linked”.
Transgenic Organisms
Overview
Cis-regulatory Sequences
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...


