单分子长读甲基化概况显示,在经历太空飞行的阿拉比多普西斯树根中,由延长者复杂子单元2调节的区域DNA甲基化
Mingqi Zhou1, Alberto Riva2, Marie-Pierre L Gauthier3
1Department of Horticultural Sciences, University of Florida, 2550 Hull Road, Fifield Hall, 32611, Gainesville, FL, USA.
Biology direct
|April 30, 2024
概括
太空飞行改变了植物DNA甲基化,一种新方法揭示了单分子分辨率的特定变化. 这发现了新的根生长调节剂,EMO1和EMO2,对于植物适应太空至关重要.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 太空生物学空间生物学
背景情况:
- 先进的植物实验-04 - 表观遗传表达 (APEX-04-EpEx) 研究了太空飞行对Arabidopsis thaliana的DNA甲基化的影响.
- 全基因组二硫酸盐测序 (WGBS) 显示了甲基化差异,但缺乏针对特定基因区域的单分子分辨率.
研究的目的:
- 开发和应用一种新的技术,用于单分子分辨率的太空飞行改变了植物的DNA甲基化.
- 为了确定受太空飞行影响的基因调节区域的特定表观遗传变化.
主要方法:
- 用式下一代捕获 (FENGC) 进行了针对性的多重复合DNA捕获和丰富.
- 分析了促进体或基因体区域中的108个目标小组的甲基化模式.
主要成果:
- FENGC提供了DNA甲基化的单分子分辨率,揭示了太空飞行改变的分布和共同甲基化趋势.
- 在三个以前未被描述的基因中发现了显著的甲基化变化,包括EMO1和EMO2.
- 功能丧失的EMO1和EMO2突变体表现出增强的根生长.
结论:
- FENGC提供了一种具有成本效益的高分辨率工厂甲基化分析方法,超越了WGBS的限制.
- 太空飞行会在单分子水平上诱导区域性细胞氨酸甲基化变化,而基于人群的方法会错过这种变化.
- 已识别的基因EMO1和EMO2是新的根生长调节剂,可能与植物适应太空飞行有关.
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Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...