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基于菌体的工程疫苗在现场重塑瘤微环境,并引起强大的抗瘤免疫力

Lei Lei^1,2, Jiayao Yan^1,2, Kai Xin³

¹The Comprehensive Cancer Centre of Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008 China.

|May 1, 2024

概括

这项研究引入了一种新的现场疫苗 (ISV),使用工程M13菌体向CD40. 这种方法增强了树突细胞 (DC) 激活,并且在结合癌症免疫疗法中显示出前景.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
纳米技术纳米技术

背景情况:

在位疫苗 (ISVs) 利用局部治疗来刺激全身抗瘤免疫力.
生物启发的ISV提供免疫辅助作用和遗传适应性.
由于其免疫性和可扩展性,M13菌体是癌症疫苗开发的有希望的天然纳米材料.

研究的目的:

开发一种基于M13菌体的基因工程ISV,针对CD40进行增强的树突细胞 (DC) 刺激.
评估H-GM-M13CD40策略与PD-1阻断结合治疗感冒瘤的疗效.

主要方法:

开发了针对CD40 (M13CD40) 的工程M13菌体.
免疫性细胞死亡是使用 (S) -10-基托丁 (HCPT) 诱导的.
进行了粒细胞-巨细胞殖民地刺激因子 (GM-CSF) 和M13CD40 (H-GM-M13CD40) 的内注射,以招募和激活DCs.

主要成果:

H-GM-M13CD40策略显著增加了瘤微环境中的DC积累和激活.
这种方法逆转了免疫抑制瘤微环境.
在冷瘤模型中,H-GM-M13CD40与PD-1阻断的结合表明了协同效应,并诱导了abscopal反应.

结论:

工程M13CD40菌体是免疫原的,适合用于癌症疫苗应用.
开发的ISV策略有效地刺激DC介导的抗瘤免疫力.
这种基于M13菌体的ISV显示出作为癌症免疫疗法的辅助剂的潜力,特别是与检查点封锁相结合.

关键词:

CD40 CD40 CD40 CD40 CD40 CD40 CD40 CD40 CD40 CD40 CD40 CD40 CD40 细菌菌体是一种细菌体.生物材料是生物材料.癌症免疫疗法免疫疗法在现场接种疫苗.

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