来自HA-b-ELP双块共聚物的热敏核心交联纳米粒子
Manon Levêque1, Sébastien Lecommandoux1, Elisabeth Garanger1
1Univ. Bordeaux, CNRS, Bordeaux INP, LCPO, UMR 5629, Pessac F-33600, France.
Biomacromolecules
|May 1, 2024
概括
我们开发了一种交叉连接方法来稳定自组装的纳米粒子,防止生物流体中的分解. 这些稳定纳米粒子在较低温度下呈现可逆胀,而不是分解.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 聚合物化学 聚合物化学
背景情况:
- 纳米粒子自组装对于药物输送和生物医学应用至关重要.
- 稀释依赖的拆卸限制了自组装纳米颗粒的体内应用.
- 稳定策略是保持在生理条件下纳米粒子完整性的必要.
研究的目的:
- 开发一种简单且生物相容的交叉连接方法,用于稳定自组装纳米粒子.
- 研究交叉链接对HA-b-ELP块共聚合物纳米颗粒的体性质和体内适用性的影响.
- 为了评估在交联纳米颗粒中热反应性行为的保留.
主要方法:
- 两性酸-阻断-胺类聚类 (HA-b-ELP) 阻断共聚合物的自组装成纳米颗粒.
- 纳米颗粒的生物相容交叉链接通过化ELP块中的氨酸残留物,使用二甲基乙烯化合物.
- 在交叉链接之前和之后,纳米粒子形态,体稳定性和热响应性行为的表征.
主要成果:
- 交叉连接反应成功地稳定了自我组装的纳米粒子,防止稀释依赖的拆卸.
- 核心交叉连接的纳米粒子保持了球形核心外形态和体稳定性.
- 交叉连接的纳米粒子保留了热反应行为,在温度下降时表现出可逆的胀和增加的水力动力直径,而不是拆解.
结论:
- 一个简单的,生物相容的交叉连接策略有效地稳定了自组装的HA-b-ELP纳米粒子.
- 稳定纳米粒子保持其结构和热反应性质,增强其在体内应用的潜力.
- 可逆膨胀机制为生物环境中控制的纳米粒子行为提供了一种新的方法.
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