设计一种非模型酵母Rhodotorula mucilaginosa,用于类化合物合成
Qiongqiong Chen1,2, Liting Lyu1,3, Haizhao Xue1,2
1Laboratory of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.
Synthetic and systems biotechnology
|May 1, 2024
概括
合成生物学使微生物能够生产有价值的类. 研究人员设计了红酵母Rhodotorula mucilaginosa以产生α-terpineol,α-farnesene和β-ionone,证明了其作为多功能生物合成平台的潜力.
科学领域:
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
- 微生物生物技术 微生物生物技术
背景情况:
- 类物质具有显著的生物活性,使其在食品,医疗保健和制药中具有价值.
- 微生物细胞工厂为传统的化物生产方法 (如化学合成或植物提取) 提供了可持续的替代方案.
- 罗多托鲁拉 (Rhodotorula mucilaginosa) 是一种红色酵母,以天然的胡卜素和脂质生产而闻名,这表明它具有强大的内源性异烯通路.
研究的目的:
- 为了设计Rhodotorula mucilaginosa菌株用于各种 terpenoids的生物合成.
- 评估R. mucilaginosa的潜力,作为生产类的微生物平台.
- 通过代谢工程策略优化类位.
主要方法:
- 用R. mucilaginosa菌株进行基因工程,以表达异构合成酶.
- 在美酸盐 (MVA) 途径内,内源性速率限制基因的过度表达.
- 使用分析方法量化类产量标位.
主要成果:
- 经过工程改造的菌株成功地产生了单二烯α-二烯醇 (0.39 mg/L) 和二烯α-二烯 (822 mg/L).
- 胡卜素降解产物β-离子子的产生达到0.87 mg/L.
- 优化MVA途径显著增强了特定类的生产.
结论:
- 罗多托鲁拉 (Rhodotorula mucilaginosa) 作为一个有前途和多功能宿主,用于各种特类的直接生物合成.
- 代谢工程方法,包括途径优化,可以大幅增加类产量.
- 这项研究为开发R. mucilaginosa作为可持续类制造工业平台提供了基础.
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