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单细胞和巨细胞中的NOX2和NOX4表达-信号和治疗中的细胞外囊泡

Deepak Rathi¹, Claudio Rossi^2,3, Pavel Pospíšil¹

¹Department of Biophysics, Faculty of Science, Palacký University, Olomouc, Czechia.

Frontiers in cell and developmental biology

|May 1, 2024

概括

细胞外囊泡 (EVs) 通过抑制单细胞中NADPH氧化酶 (NOX) 酶分化为巨细胞,作为抗氧化剂. 本研究描述了EV及其在调节氧化应激和NOX异型中的作用.

科学领域:

细胞生物学细胞生物学
免疫学免疫学免疫学
生物化学生物化学

背景情况:

细胞外囊泡 (EVs) 是分泌的囊泡,参与细胞间通信和免疫反应.
电动汽车可以携带活性尼古丁胺亚丁氨酸丁核酸氧化酶 (NADPH) 氧化酶,这对于反应性氧物种 (ROS) 生产至关重要.
NADPH氧化酶 (NOX) 活动影响细胞再生和氧化应激.

研究的目的:

来自U-937和THP-1细胞的EV的特征.
在这些EV中识别NADPH氧化酶 (NOX) 异型.
为了确定EV是否调节单细胞和巨细胞中的NOX4和NOX2表达.

主要方法:

从U-937细胞中分离的EVs使用动态光散射 (DLS) 和免疫阻塞进行了表征.
单细胞 (U-937和THP-1) 与EVs共同培养.
氧化应激水平使用免疫阻塞和电子磁共振 (EPR) 谱学进行了评估.

主要成果:

EVs没有诱导细胞应激或影响增殖/活力.
与EVs共同培养在单细胞到巨细胞分化过程中显著抑制了NOX4和NOX2.
电动汽车共同培养导致氧化应激减少,这表明电动汽车具有抗氧化作用.

结论:

在单细胞分化过程中,EVs在调节NOX异型中发挥作用,特别是NOX4和NOX2.
电动汽车具有抗氧化特性,可能充当治疗剂.
这些发现凸显了EVs在细胞过程和氧化还原平衡中的多方面的作用.

关键词:

在NADPH氧化酶.氧二氧化 (NOX2) 是一种在NOX4中,NOX4是细胞外囊泡中的细胞外囊泡.自由基是自由基的重要组成部分.巨细胞是什么?巨细胞是什么?单细胞 (Monocytes) 是一种单细胞.有活性氧物种的反应性氧物种.

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