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识别长非编码RNAs对丝虫,Bombyx mori中的微球菌感染的反应

Shiqi Pu¹, Yujia Fang¹, Yuhang Yang¹

¹Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.

Journal of economic entomology

|May 1, 2024

概括

这项研究揭示了丝长非编码RNAs (lncRNAs) 如何应对Nosema bombycis (Nb) 感染. 研究人员确定了参与新陈代谢和免疫反应的关键lncRNAs,为Pébrine疾病提供了洞察力.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
昆虫病理学昆虫病理学

背景情况:

虫 (Nosema bombycis) (Nb) 在丝虫中引起虫病,影响丝种植.
长非编码RNAs (lncRNAs) 在细胞功能中起着至关重要的作用,但它们在丝虫对Nb感染的反应中的作用尚不清楚.

研究的目的:

为了研究丝虫 lncRNAs 在对 Nosema bombycis 感染的反应中的作用.
识别差异表达的lncRNA和mRNA并分析它们的功能和调节网络.

主要方法:

丝虫幼虫和Nb感染的中肠的转录组测序.
对差异表达的lncRNAs (DElncRNAs) 和mRNAs (DEmRNAs) 的鉴定和分析.
同表达网络和DElncRNA-miRNA-mRNA网络的构建.

主要成果:

鉴定了1,440个lncRNA;在Nb感染的丝虫中检测到42个DElncRNA和305个DEmRNA.
德米RNAs主要参与新陈代谢,亡和自途径.
观察到涉及lncRNAs,miRNAs和mRNAs的复杂调节网络,其中MSTRG857.1可能促进Nb的扩散.

结论:

丝虫lncRNAs与Nb感染的反应密切相关.
lncRNAs可以通过miRNA途径调节免疫反应和新陈代谢.
特定的lncRNAs,如MSTRG857.1,可能是Nb扩散和蛋白疾病发展的关键参与者.

关键词:

博姆比克斯 (Bombyx mori mori mori) 是一个死神.诺塞马的轰炸就是轰炸.在 lncRNA 中,在cnRNA-miRNA-mRNA中.

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