新兴的抗生素持久性在一个空间结构合成微生物互惠主义
Xianyi Xiong1,2, Hans G Othmer3, William R Harcombe1
1Department of Ecology, Evolution, and Behavior, BioTechnology Institute, University of Minnesota, St. Paul, MN 55108, United States.
The ISME journal
|May 1, 2024
概括
微生物群落可以增强抗生素的持久性. 在两种系统中,交叉养和空间结构增加了抗生素耐受性大肠杆菌的55倍,突出了细菌生存中的生态因素.
科学领域:
- 微生物学 微生物学
- 生态生态学 生态生态学
- 进化生物学 进化生物学
背景情况:
- 抗生素持久性 (异种耐受性) 使细菌亚群能够在抗生素治疗中存活下来,这有助于耐药性进化.
- 微生物生态和社区相互作用在抗生素持久性中的作用在很大程度上仍未被探索.
研究的目的:
- 研究微生物生态,特别是合成社区的交叉养和空间结构如何影响抗生素的持久性.
- 确定物种间相互作用对在抗生素压力下细菌生存的新兴影响.
主要方法:
- 采用了合成的两种类型互惠性大肠杆菌和沙门氏菌.
- 使用时间序列光显微镜追踪了阿加表面上安培素诱导的细菌死亡.
- 量化抗生素在单种植和共同种植中持续形成.
主要成果:
- 与单一培养相比,与沙门氏菌的交叉养共同培养中,大肠杆菌形成的抗生素持续性高达55倍.
- 高持久性是一种新兴现象,不仅仅由物种存在,交叉养,营养水平或突变来解释.
- 在互惠共养殖中,在大肠杆菌中观察到延迟时间中细胞对细胞的变化增加.
结论:
- 微生物群落中交叉养和空间结构的结合可以突发性地驱动高抗生素持久性.
- 空间结构的相互作用和生态因素对于理解细菌弹性和抗生素治疗结果至关重要.
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