由内共生细菌产生的Deazaflavin代谢物控制着真菌宿主繁殖
Ingrid Richter1, Mahmudul Hasan2, Johannes W Kramer1
1Department of Biomolecular Chemistry, Leibniz Institute for Natural Product Research and Infection Biology (Leibniz-HKI), 07745 Jena, Thuringia, Germany.
The ISME journal
|May 1, 2024
概括
菌群Mycetohabitans rhizoxinica产生FO,这是3PG-F420辅因子的前体,对于菌群Rhizopus microsporus来说是必不可少的,以化并维持共生. 这突出了FOFOFO.
科学领域:
- 微生物学 微生物学
- 共生研究是对共生的研究.
- 菌菌与细菌的相互作用
背景情况:
- 里索普斯微粒体和Mycetohabitans rhizoxinica之间的内共生包括依赖细菌内共生体和二次代谢物的真菌分泌.
- 主代谢物,特别是由M. rhizoxinica产生的FO和3PG-F420在这种宿主-内共生关系中的作用以前未被探索.
研究的目的:
- 研究FO和3PG-F420在R. microsporus和M. rhizoxinica之间的共生关系中的作用.
- 确定FO是否对于建立稳定和功能共生至关重要.
主要方法:
- 对M. rhizoxinica基因组的生物信息分析,以确定参与3PG-F420生产的基因.
- 开发一种CRISPR/Cas辅助的基编辑策略,以创建缺乏3PG-F420 (ΔcofC) 和FO和3PG-F420 (ΔfbiC) 的M. rhizoxinica突变菌株.
- 与阿波共生R. microsporus和M. rhizoxinica菌株的共同培养实验,以及超分辨率光显微镜和遗传/化学补充测试.
主要成果:
- 基因分析证实了3PG-F420生物合成基因在内菌Mycetohabitans菌株中的保存.
- 尽管成功殖民,但R. microsporus Sporulation与野生型M. rhizoxinica和ΔcofC突变物保持在一起,但与ΔfbiC突变物没有保持在一起.
- 在M. rhizoxinica中补充FO缺乏 ΔfbiC恢复了真菌分泌,表明FO的关键作用.
结论:
- 作为3PG-F420的前体,FO对于在R. microsporus和M. rhizoxinica.之间建立功能共生是不可或缺的.
- 这项研究揭示了FO在王国间通信中的重要作用,超出了其已知的光采集特性.
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