细胞壁介导的根部发育是土壤传播的细菌病原体的目标,以促进感染
1Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai 201602, China; Shanghai Collaborative Innovation Center of Agri-Seeds, Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
细菌效应剂RipAC针对植物中的细胞合成酶 (CESA) -交互性蛋白1 (CSI1). 这种相互作用破坏了纤维素合成,改变了根的发育,促进了Ralstonia solanacearum感染,而不会触发植物免疫力.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 植物开发 植物开发
背景情况:
- 植物病原体经常操纵宿主发育以建立感染.
- 拉尔斯托尼亚 (Ralstonia solanacearum) 是一种破坏性土壤传播的细菌,通过殖民血管系统,在植物中引起枯疾病.
研究的目的:
- 研究Ralstonia solanacearum效应蛋白RipAC影响宿主植物发育的分子机制.
- 确定RipAC的宿主目标,并了解它们对细菌致病性有何贡献.
主要方法:
- 研究了RipAC对Arabidopsis thaliana根结构的影响.
- 使用共免疫沉试验来识别与RipAC的宿主蛋白相互作用.
- 分析了纤维素含量及其与根部发育和细菌感染的相关性.
主要成果:
- 在Arabidopsis中,RipAC促进横向根和根毛发的形成.
- RipAC与纤维素合成 (CESA) - 交互蛋白1 (CSI1) 直接相互作用,这是纤维素合成的调节者.
- 破坏RipAC-CSI1相互作用会降低纤维素含量,改变根的发育,并增强R. solanacearum的致病性.
- RipAC不会影响CSI1与免疫相关受体激酶FERONIA之间的相互作用.
结论:
- RipAC采用了一种新的毒性策略,通过选择性地准参与细胞壁合成的宿主因素.
- 由RipAC诱导的根部发育的改变促进了病原体的殖民,而没有激活植物的免疫反应.
- 了解这些病原体与宿主相互作用,可以了解植物防御机制以及疾病控制的潜在策略.
更多相关视频
11:50Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
Published on: October 1, 2015
11:16A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
相关概念视频
Plant Cell Wall
Defenses Against Pathogens and Herbivores
Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Water and Mineral Acquisition
Bacterial Signaling
Receptor-mediated Endocytosis
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...
