从废水中获取新兴病毒的探测捕获丰富测序的考虑和机会
1Department of Civil and Environmental Engineering, University of California, Berkeley, Berkeley, California 94720, United States.
Environmental science & technology
|May 1, 2024
概括
使用测序和探针丰富的废水病原体监测显示出追踪疾病和检测新威胁的前景. 优化方法是提高病原体检测准确度和减少错误的关键.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 公共卫生监督是对公共卫生的监督.
背景情况:
- 基于废水的监测传统上依赖PCR和针对特定病原体的定向测序,如SARS-CoV-2.
- 最近的进展包括扩展测序与探头捕获丰富用于废水中的广泛病原体监测.
研究的目的:
- 监测已知病原体的疾病动态.
- 评估从动物传播传播或新型变异中新出现的疾病的早期检测潜力.
主要方法:
- 在废水样本上应用探头捕获丰富技术.
- 分析用于临床和环境监测的现成探针面板.
- 评估废水处理,探测板设计和生物信息分析.
主要成果:
- 富化增加了病毒的相对丰富性,但往往无法恢复非肠道病毒的完整基因组.
- 研究发现了在废水病原体检测中影响虚假阳性和虚假阴性率的挑战.
- 在整个工作流程中存在权衡和机会.
结论:
- 建议采用定制的病毒度和背景清除方法.
- 建议精心设计的探针面板和多门生物信息学分析,以提高准确性.
- 优化这些因素对于可靠的基于废水的病原体监测至关重要.
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