欧米克斯分析显示,阿弗拉托xin B1 和阿弗拉托xin M1 诱导的肠道毒性
Ya-Nan Gao1, Zi-Wei Wang2, Chuan-You Su3
1Key Laboratory of Quality & Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
暴露于阿弗拉托克素B1和阿弗拉托克素M1会损害小鼠的肠道屏障. 对这些真菌毒素的联合暴露会比单个暴露造成更大的伤害,影响脂质代谢和细胞完整性.
科学领域:
- 毒理学 毒理学 毒理学
- 蛋白质组学是指蛋白质组学.
- 胃肠病学 胃肠病学
背景情况:
- 甲素B1 (AFB1) 和其代谢物甲素M1 (AFM1) 是分别在农产品和乳制品中发现的真菌毒素.
- 这些毒素对健康构成风险,但它们对肠道屏障功能的具体影响仍然在很大程度上不明.
研究的目的:
- 研究AFB1和AFM1单独或组合对小鼠肠道屏障功能的影响.
- 通过蛋白质组学分析,识别差异表达蛋白 (DEPs) 和受这些真菌毒素影响的相关途径.
主要方法:
- 鼠被暴露在AFB1和AFM1 (单独或组合) 中28天.
- 在小鼠样本上进行蛋白质组分析,以确定DEP和丰富的途径.
- 这些发现初步通过Caco-2细胞的转录组和蛋白组分析进行了验证.
主要成果:
- AFB1和AFM1都破坏了肠道屏障功能,联合暴露显示出更大的毒性.
- AFB1诱导的DEP丰富了脂质代谢途径.
- AFM1诱导的DEP刺激了蛋白质出口路径.
- 联合治疗抑制了细胞代谢能力,诱导了细胞亡,并破坏了上皮细胞的完整性.
结论:
- 蛋白质组分析揭示了AFB1和AFM1.1对肠道屏障破坏的独特和协同机制.
- 对AFB1和AFM1的联合暴露对肠道健康产生重大危险影响.
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