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功能性光探针的敏感分析策略,用于检测人体血清中的nusinersen

Yujuan Zhan¹, Jingru Guo¹, Penghui Hu¹

¹Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 210009, China; Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, Nanjing, 211198, China.

|May 1, 2024

概括

一种新方法使用磁纳米粒子和光探针进行超灵敏检测nusinersen,一种用于脊髓肌肉缩的抗意义寡核类药物. 这种方法可以在人血清中进行快速和具体的分析,有助于治疗药物监测.

科学领域:

生物化学生物化学
分析化学分析化学
纳米技术纳米技术

背景情况:

脊椎肌肉缩 (SMA) 是一种罕见的遗传神经肌肉疾病.
努辛森 (NS) 是对SMA的第一类反感性寡核酸 (ASO) 疗法.
在ASO药物监测方面的挑战包括短半衰期和组织积累.

研究的目的:

开发一种超灵敏,高通量分析方法,用于人类血清中nusinersen.
克服当前ASO药物监测技术的局限性.
为基于ASO的疗法提供精确的治疗药物监测.

主要方法:

使用单链结合蛋白 (SSB) 功能化磁纳米粒子 (MNPs-SSB) 进行选择性捕获.
采用红色光量子点 (RQDs-ssDNA) 功能化的寡核酸,以补充努辛森.
开发了一种基于光的测定方法,加上用于定量分析的磁性分离.

主要成果:

实现了对nusinersen的超敏感检测,检测极限 (LOD) 为0.03nM.
证明了从0.1nM到200nM (R2 = 0.9871) 的广泛线性检测范围.
该试验提供了高特异性和快速分析时间 (30分钟).

结论:

拟议的MNP-SSB和RQDs-ssDNA战略为ASO药物分析提供了一种新的方法.
这种方法有助于在生物样本中敏感,高吞吐量和特定检测努辛森.
这些发现支持改善ASO治疗的治疗药物监测.

关键词:

光探测器探测器可以发光.人类血清的人类血清努辛森纳的使用方法单链结合蛋白质是一种单链结合蛋白质.这就是 ssDNADNA.

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