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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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用基于氨酸的光探针进行氧化物捐赠和可视化.

Jiajun Chen1, Yunxi Cui2, Peixuan Wu1

  • 1Key Laboratory of Advanced Materials of Tropical Island Resources of Ministry of Education and School of Chemical Engineering and Technology, Hainan University, Haikou, Hainan 570228, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
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概括

研究人员开发了一种基于素的新型化合物 (CD1),可以释放氧化物 (HNO) 和光信号. 这种双重功能捐赠器允许实时监测生物系统中HNO释放的情况.

关键词:
细胞成像 细胞成像提供者分子分子.光是一种光效应.氧化 (HNO) 是一种探测器探测器探测器

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科学领域:

  • 化学 化学 化学
  • 生物医学工程 生物医学工程
  • 药理学 药理学是指药理学的学科.

背景情况:

  • 氧化 (HNO) 是一种信号分子,对充血性心力衰竭等疾病具有治疗潜力.
  • 现有的检测HNO释放的方法可以在实时监测和生物应用方面受到限制.
  • 开发新的HNO捐赠者对于推动与HNO相关的研究和治疗策略至关重要.

研究的目的:

  • 合成和表征一种新的基于库马林的化合物 (CD1) 作为双功能的HNO供体.
  • 在生理条件下研究 CD1 的 HNO 释放动力学和静电学.
  • 为了证明CD1在HNO和细胞内生物成像的现场监测中的实用性.

主要方法:

  • 合成N-基-2-oxo-2H-基-6-硫胺 (CD1) 的合成.
  • 在pH7.4和37°C下对CD1进行分解研究,以确定半衰期.
  • 使用维生素B12和氨酸陷确认HNO静脉测量.
  • 降解产品 (CS1) 的光监测用于HNO量化.
  • 在HeLa细胞中进行细胞生物成像,以可视化细胞内HNO释放.

主要成果:

  • 在生理条件下,CD1的半衰期约为90分钟.
  • 证实了从CD1中释放HNO的静脉测量释放.
  • 降解产物2-oxo-2H-chromene-6-sulfinate (CS1) 作为HNO的可靠光标记物.
  • 光强度增加4.9倍与HNO释放相关,检测极限为~0.13μM.
  • 在HeLa细胞中观察到细胞内光信号,表明HNO释放的成功生物成像.

结论:

  • 这种基于氨酸的新型CD1化合物作为一种有效的双功能HNO捐赠体.
  • CD1允许通过光生成准确的实时监测HNO释放.
  • CD1有望成为探索HNO生物学和潜在治疗应用的有价值工具.