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基于废水的监测是一种有效的监测工具,用于在社区中追踪A型流感
Kirsi-Maarit Lehto1, Annika Länsivaara1, Rafiqul Hyder1
1Tampere University, Faculty of Medicine and Health Technology, Arvo Ylpön katu 34, Tampere 33520, Finland.
Water research
|May 1, 2024
概括
废水监测有效地监测了流感A病毒 (IAV) 在人群中的RNA水平. 这种方法与疾病病例相关,提供了成本效益高的公共卫生准备工具.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 公共卫生监督是对公共卫生的监督.
背景情况:
- A型流感病毒 (IAV) 构成流行病风险,目前的监测依赖于临床数据.
- 现有的监测方法可能很少,并且落后于实际的病毒循环.
研究的目的:
- 评估基于废水的监测,以监测人口层面的IAV RNA.
- 评估废水IAVRNA水平与报告的疾病病例之间的相关性.
主要方法:
- 分析了来自10个处理厂的251个每月的废水流量样本,覆盖芬兰40%的人口 (2021年2月至2023年2月).
- 使用特定的逆转录定量聚合酶链反应 (RT-qPCR) 方法检测上浮子部分中的IAVRNA.
主要成果:
- 流感A病毒RNA在废水中始终可检测到.
- 废水IAV RNA基因拷贝数与确诊的疾病病例有显著的正相关性 (Kendall的中位数t = 0.636).
- 这种相关性在所有10个监测的废水处理厂流域中都具有统计学意义.
结论:
- 基于废水的监测提供了一种独立且具有成本效益的方法,用于跟踪人口层面的IAV循环.
- 这种方法补充了传统的监测,提高了对IAV爆发和流行病的准备.
- 废水监测为公共卫生传染病动态提供了宝贵的见解.
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