相关实验视频
Updated: Jun 27, 2025

08:08
Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines
Published on: June 1, 2022
2.1K
沃尔巴基亚共生体在寄生虫黄蜂中使用水平获得的基因控制性
Ce Li1, Chu-Qiao Li1, Zhan-Bo Chen1
1Liaoning Key Laboratory of Economic and Applied Entomology, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Current biology : CB
|May 1, 2024
概括
细菌共生体通过伴生代操纵宿主繁殖. 这项研究确定了一种Wolbachia基因,该基因会改变昆虫的性别决定,从而诱导黄蜂的雌性生产性生.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 进化生物学 进化生物学
背景情况:
- 细菌共生体可以操纵宿主繁殖,其中伴生诱导是共生体传播的关键.
- 昆虫的性别决定依赖于通过变压器 (tra) 和变压器2 (tra2) 相互作用调节双性 (dsx) 拼接.
- 在共生体诱导的 partenogenesis 背后的分子机制在很大程度上是未知的.
研究的目的:
- 确定细菌共生体Wolbachia在寄生虫黄蜂Encarsia formosa中诱导 partenogenesis的分子机制.
- 调查沃尔巴基因在操纵宿主性别决定途径中的作用.
主要方法:
- 在Encarsia formosa.中进行了Wolbachia消除实验.
- 对宿主性别决定基因 (dsx,tra,tra2) 的基因表达分析.
- 鉴定和描述具有潜在的性别决定功能的新型沃尔巴基因 (piff).
- 对Wolbachia PIFF与宿主TRA2之间的蛋白相互作用进行分析.
主要成果:
- 沃尔巴基亚的消除逆转了性别决定,导致男性后代的产生.
- 一个沃尔巴基亚基因,伴生诱导女性化因子 (piff),被确定为昆虫的功能同类物.
- 沃尔巴基亚PIFF与宿主TRA2相互作用,并与宿主性别决定基因共同表达,操纵级联诱导女性偏向的生.
结论:
- 细菌共生体Wolbachia已经获得了一种昆虫基因 (tra homolog,piff) 来操纵宿主的性别决定并诱导伴生.
- 这种从昆虫到细菌的横向基因转移推动了动物性别确定系统的进化.
- 这项研究阐明了一种昆虫微生物共生和宿主生殖操纵的新机制.
相关概念视频
Dosage Compensation
6.2K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.2K
The Ratio of X Chromosome to Autosomes
8.5K
In most organisms, sex is determined by the ratio of X and Y chromosomes. However, in some organisms, such as Drosophila and C.elegans, sex is determined by the ratio of the number of X chromosomes to the number of sets of autosomes. The Y chromosome in Drosophila is active but does not determine sex. It contains genes responsible for the production of sperms in adult flies.
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female...
8.5K
Pleiotropy
40.4K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
40.4K
Types of Genetic Transfer Between Organisms
27.8K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
27.8K
Symbiosis
27.9K
Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...
27.9K

