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Wenhao He¹, Jinji Lin¹, Feifei Yu²

¹College of Fisheries, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China; Academician Joint Laboratory of Germplasm Resource Exploitation, Utilization and Health Assessment for Aquatic Animal, Guangdong Ocean University, Zhanjiang, Guangdong, 524088, China.

Fish & shellfish immunology

|May 1, 2024

概括

这项研究在Pteria企中发现了一种新的BCL2基因,对免疫防御至关重要. 它的表达在Vibrio parahaemolyticus感染期间增加,突出了它在打击细菌挑战中的作用.

科学领域:

海洋生物学海洋生物学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

B细胞淋巴瘤/白血病-2 (BCL2) 基因是调节免疫反应的关键抗亡因子.
了解BCL2在无脊椎动物中的作用对于免疫防御机制至关重要.

研究的目的:

为了表征一种来自Pteria企 (PpBCL2) 的新型BCL2基因.
为了研究PpBCL2在P.企对Vibrio parahaemolyticus的免疫反应中的功能.

主要方法:

克隆和测序Pteria企BCL2 (PpBCL2) 的基因.
生物信息分析包括氨基酸对齐和家族遗传树结构.
通过RNA干扰进行基因沉默,并评估抗微生物活性和感染后的生存率.

主要成果:

发现了一种新型的PpBCL2基因,1482bp,ORF为588bp,编码195个氨基酸,具有4个保存的BCL-2同源域.
PpBCL2与来自Crassostrea gigas的BCL2的相似性最高 (65.24%) .
PpBCL2在免疫组织 (,消化分泌体,地幔) 中具有构成性表达,并在Vibrio parahaemolyticus挑战时显著上调.
沉默PpBCL2降低了血淋巴抗菌活性1.4倍,并在感染72小时后降低了51.7%的存活率.

结论:

PpBCL2在Pteria企对抗Vibrio parahaemolyticus感染的免疫防御中发挥着重要作用.
这项研究提供了无脊椎动物免疫的分子机制的见解,涉及BCL2家族蛋白质.

关键词:

在BCL2中,BCL2是免疫反应的免疫反应.斑点企 (罗丁,1798) 在 1798 年.V. parahaemolyticususus 这种类型的血清过分.

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