在DPSC上的纸封装材料的生物相容性和细胞毒性,具有标记器mRNA表达
Banu Çiçek Tez1, Bahar Başak Kızıltan Eliaçık2, Pakize Neslihan Taşlı3
1Department of Pediatric Dentistry, Faculty of Dentistry, Ankara Medipol University, Ankara, Türkiye.
International dental journal
|May 1, 2024
概括
哈佛BioCal-Cap和NeoPUTTY MTA表现出极好的生物相容性,并支持人类牙纸干细胞 (hDPSC) 中的血管原标记表达. 这些发现突显了它们作为有效的纸封装材料的潜力.
科学领域:
- 牙科材料科学 牙科材料科学
- 干细胞生物学 干细胞生物学
- 恢复性牙科 恢复性牙科
背景情况:
- 纸覆盖材料对于保持牙纸活力至关重要.
- 评估这些材料的生物相容性和再生潜力对于成功的临床结果至关重要.
- 人类牙纸干细胞 (hDPSCs) 是纸修复和再生的关键细胞组成部分.
研究的目的:
- 评估hDPSC上的四种纸封装材料的生物相容性和细胞毒性.
- 为了确定这些材料对血管新生,口腔新生和骨质新生标记基因表达的影响.
- 使用扫描电子显微镜 (SEM) 检查hDPSCs暴露于封装材料时的形态变化.
主要方法:
- 从受影响的第三牙中分离出hDPSCs,并使用哈佛BioCal-Cap,NeoPUTTY MTA,TheraCal LC和Dycal.的提取释放液体进行治疗.
- 使用MTS试验评估了细胞活力,通过流细胞计确定了细胞亡/亡细胞比率和活性氧物种 (ROS) 水平.
- 关键标记物的基因表达 (ALP,OCN,Col1A,SPARC,ON,VEGF) 使用RT-qPCR量化,细胞形态通过SEM可视化.
主要成果:
- 哈佛BioCal-Cap,NeoPUTTY MTA和TheraCal LC在第7天之前没有对hDPSC生存能力产生不良影响.
- 迪卡尔是唯一能够显著增加ROS水平的材料.
- 哈佛大学的BioCal-Cap,TheraCal LC和NeoPUTTY MTA促进了高VEGF表达,表明了血管生成潜力. NeoPUTTY MTA和Dycal上调了OCN,而TheraCal LC则上调了Col1A和SPARC. 这两种类型的药物具有很大的影响. SEM揭示了与哈佛BioCal-Cap和TheraCal LC治疗的hDPSC的特征性形态.
结论:
- 哈佛大学的BioCal-Cap和NeoPUTTY MTA与hDPSCs具有良好的生物相容性.
- 这些材料显示出支持血管新生过程的潜力,这对于纸修复至关重要.
- 该研究提供了宝贵的见解hDPSCs对不同纸封装剂的生物反应.
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