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RUNX1/NF-κB在调节大动脉解剖中的PVAT炎症中的作用
Ao Wang1, Shengjun Dong1, Baohui Liu1
1Department of Cardiovascular Surgery, Binzhou Medical University Hospital, Binzhou, 256600, Shandong Province, China.
Scientific reports
|May 1, 2024
概括
周血管脂肪组织 (PVAT) 炎症与大动脉剖析 (AD) 有关. RUNX1通过RUNX1/NF-κB通路调节PVAT炎症,这表明它是AD的潜在治疗标.
科学领域:
- 心血管生物学 心血管生物学
- 血管炎症 血管炎症
- 脂肪组织生物学 脂肪组织生物学
背景情况:
- 大动脉解剖 (AD) 是一种危及生命的疾病,其特点是血管炎症.
- 周周脂肪组织 (PVAT) 在AD病变发生过程中的作用尚未完全理解.
- 确定阿尔茨海默病的新型治疗点至关重要.
研究的目的:
- 为了研究PVAT炎症在AD病变发生中的作用.
- 通过检查PVAT-AD关系来确定AD的潜在治疗点.
- 为了阐明RUNX1在PVAT与AD相关的炎症变化中的参与.
主要方法:
- 使用Ang II和BAPN建立了AD的小鼠模型.
- 利用qPCR和免疫组织化学分析小鼠和人类PVAT中的炎症标志物和RUNX1表达.
- 使用3T3-L1脂肪细胞和与巨细胞和血管光滑肌细胞共同培养系统进行的体外研究.
- 评估了RUNX1淘汰赛小鼠对AD指标的评估.
主要成果:
- 在AD模型小鼠中,TNF-α和RUNX1增加,MMP-2和NF-κB在PVAT中减少.
- 在脂肪细胞中的RUNX1操纵改变了NF-κB,MMP-2和TNF-α水平.
- 在脂肪细胞中RUNX1过度表达影响了巨细胞 (CD86) 和血管光滑肌细胞 (α-SMA) 标记物.
- 在阿尔茨海默病患者中,人类样本证实了RUNX1和MMP-2的增加,TNF-α和NF-κB的减少.
结论:
- PVAT在AD中表现出显著的炎症性变化,与RUNX1.1相关.
- RUNX1/NF-κB信号通路似乎可以调节AD病变的PVAT炎症.
- RUNX1是一种潜在的治疗点,用于治疗大动脉剖析.
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