在匈牙利的儿童游乐场中分离的金黄色葡萄球菌的高克隆多样性
Andrea Horváth1, Áron Tormássi2, Szofia Hajósi-Kalcakosz3
1Institute of Medical Microbiology, Semmelweis University, Nagyvárad tér 4., 1089, Budapest, Hungary. horvath.andrea1@semmelweis.hu.
Scientific reports
|May 1, 2024
概括
在匈牙利的游乐场上发现了Staphylococcus aureus,一种常见的细菌. 虽然没有检测到MRSA,但分离物体表现出抗生素耐药性,并携带各种毒性基因,突出显示户外环境.
科学领域:
- 环境微生物学环境微生物学
- 细菌病原发生的细菌.
- 公共卫生微生物学
背景情况:
- 金黄色葡萄球菌 (Staphylococcus aureus) 是一个重要的人类病原体.
- 环境表面,包括儿童游乐场,可以促进它的传播.
- 关于欧洲游乐场S. aureus流行情况的数据有限.
研究的目的:
- 调查匈牙利游乐场S. aureus的流行率和特征.
- 分析孤立的金黄色菌株的抗生素敏感性和毒性因素.
- 为了确定在这个环境中发现的金黄色菌的克隆多样性.
主要方法:
- 从匈牙利16个城市的游乐场收集了355个样本.
- 测试了黄金菌的存在,抗生素敏感性 (9种抗生素) 和毒性基因 (PCR).
- 使用脉冲场凝电泳 (PFGE) 和多位序列类型 (MLST) 分析了克隆多样性.
主要成果:
- 黄金菌的流行率为2.81% (10/355);没有检测到抗甲素的黄金菌 (MRSA).
- 鉴定了各种毒性基因 (spa,fnbA,icaA,cna,sea,hla,hlb,hlg) 的发现.
- 隔离物表现出多种 PFGE 脉型,属于多种 MLST 序列类型 (ST),包括一种新型 ST6609.
结论:
- 黄金菌存在于匈牙利的游乐场上,特别是在塑料表面.
- 这些分离物具有克隆的多样性,并且对常见抗生素表现出耐药性.
- 这些发现强调了户外环境在社区S. aureus传播中的作用.
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