Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

RNA-Protein complexes and their role in cell fate.

Physiology (Bethesda, Md.)·2026
Same author

Photo-triggered Formation of an Amidinyl Radical from O-Nitroveratril Amidoxime and Its Intramolecular Cyclization in Aqueous Media.

The Journal of organic chemistry·2026
Same author

RNA-binding landscape of amiloride: large-scale profiling and structural basis of U-U mismatch recognition.

RSC chemical biology·2026
Same author

Singlet oxygen-mediated photocatalytic generation of abasic sites in DNA.

Communications chemistry·2026
Same author

Systematic identification of variant-specific RNA structure-small molecule interactions exemplified by RNA G-quadruplexes.

Nature communications·2026
Same author

"More" Artificial mRNAs: Beyond the Art of Nature.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

相关实验视频

Updated: Jun 27, 2025

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.1K

使用多重RNA结构库对小分子-RNA相互作用进行大规模分析.

Ryosuke Nagasawa1,2, Kazumitsu Onizuka3,4,5, Kaoru R Komatsu6

  • 1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Miyagi, 980-8577, Japan.

Communications chemistry
|May 1, 2024
PubMed
概括

一个新的系统能够对小分子-RNA相互作用进行大规模分析,分析结合场景,并促进针对性治疗的新型RNA结合分子的发现.

更多相关视频

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.6K
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

3.6K

相关实验视频

Last Updated: Jun 27, 2025

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

12.1K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.6K
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
07:55

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Published on: February 17, 2023

3.6K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 药物发现 药物发现 药物发现

背景情况:

  • 了解小分子-RNA相互作用对于开发RNA向疗法至关重要.
  • 选择性和结合性质是设计RNA结合分子的关键挑战.

研究的目的:

  • 开发和验证一种用于大规模分析小分子-RNA相互作用的新系统.
  • 描述特定小分子的结合场景,并证明该系统在发现新的RNA结合剂方面的实用性.

主要方法:

  • 使用多重拉下测试与RNA结构库相结合,用于高通量选.
  • 采用G-clamp和 thiazole色衍生物来探测RNA结构并验证试验.
  • 集成光指示器位移 (FID) 测试用于选RNA结合分子.

主要成果:

  • 成功地描述了G-clamp和 thiazole色衍生物的RNA结合场景.
  • 根据大规模的亲缘关系概况,确定了这些分子的结合偏好.
  • 在FID测定中使用高级和中级RNA的可靠查结果.

结论:

  • 开发的系统提供了对小分子-RNA相互作用的基本见解.
  • 该平台有助于有效地发现用于治疗应用的新型RNA结合分子.
  • 这项研究强调了RNA结构库在理解分子识别方面的重要性.