与SARS-CoV-2感染相关的眼部A-to-IRNA编辑签名
Yun-Yun Jin1,2,3,4, Ya-Ping Liang1,2,3,4, Wen-Hao Huang1,2,3,4
1Laboratory of Genomic and Precision Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China.
BMC genomics
|May 1, 2024
概括
COVID-19 感染改变了眼部RNA编辑,影响了参与免疫和细胞过程的基因. 这项研究揭示了在五个眼组织中腺转化为 inosine (A-to-I) RNA 编辑的显著变化,为眼科并发症提供了洞察力.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 眼科医生 眼科 眼科
背景情况:
- 眼科表现越来越多地在COVID-19患者中被识别出来.
- 主体RNA编辑与病毒感染有关,但其在SARS-CoV-2感染期间在眼组织中的作用尚不清楚.
研究的目的:
- 在SARS-CoV-2感染期间,在五个眼组织中研究腺转化为 inosine (A-to-I) RNA 编辑.
- 了解差异性RNA编辑 (DRE) 在COVID-19背景下对基因功能的影响.
主要方法:
- 从结膜,四肢,角膜,膜和视网膜器官中取37个样本进行了表皮转录分析.
- 对A-to-IRNA编辑水平和相关基因的分析.
- 差异编辑基因的功能丰富分析.
主要成果:
- 在所有五个眼组织中,SARS-CoV-2感染显著改变了A-to-IRNA编辑.
- 角膜RNA编辑增加,而其他组织的总体下降.
- DRE在与蛋白质代谢,转录调节和RNA剪接相关的基因中占据了突出地位,包括先天性免疫基因MAVS和MDM2.
结论:
- RNA编辑在COVID-19相关的眼科症状中发挥着潜在的作用.
- SARS-CoV-2 感染通过眼部组织的转录组变化影响先天免疫力.
- 在感染期间,随着时间的推移,在视网膜器官中观察到动态RNA编辑变化.
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