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乳腺癌与淋巴结转移的异染色体圆形DNA景观

Yuhan Bao¹, Xiaolong Sui², Xiaofei Wang³

¹Breast Center, The Second Hospital of Shandong University, Jinan, China.

International journal of cancer

|May 2, 2024

概括

异染色体圆形DNA (eccDNA) 在乳腺癌转移中起作用. 研究人员在原发性瘤和淋巴结中发现了eccDNA,一些miRNA相关的eccDNA在转移性组织中得到丰富,这表明它在癌症扩散中的作用.

科学领域:

在瘤学瘤学.
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

乳腺癌 (BC) 是一种异质性疾病,其中淋巴结转移 (LNM) 显著影响患者的结果.
异染色体圆形DNA (eccDNA) 与各种癌症有关,但其在BC LNM中的特定作用尚不清楚.

研究的目的:

为了调查eccDNA在原发性乳腺癌组织和匹配的淋巴结转移中的存在和特征.
探索eccDNA,特别是miRNA相关eccDNA在乳腺癌转移过程中的潜在作用.

主要方法:

循环-Seq分析对19个BC患者的原发性瘤和配对的LNM组织进行.
鉴定eccDNA的特征包括量化,尺寸分布,重复序列分析,GC含量和侧面图案识别.

主要成果:

在原发性瘤 (中位数为44,682) 和LNM组织 (中位数为38,057) 中检测到高丰度的eccDNA.
这两种组织类型的eccDNA具有相似的尺寸分布,重复序列,GC含量和palindromic三核酸侧面图案,表明它们具有共同的染色体起源.
一个miRNA相关的eccDNA子集,包括miR-6730和miR-548AA1的子集,在转移性病变中显示有选择性的丰富.

结论:

初级乳腺瘤中的eccDNA分子及其相应的淋巴结转移具有相似的基因组起源.
转移性组织中特定的miRNA相关eccDNA的选择性丰富表明它们在乳腺癌转移级联中的功能参与.
这些发现凸显了eccDNA作为乳腺癌转移中的生物标志物或治疗点的潜在意义.

关键词:

乳腺癌乳腺癌乳腺癌这就是为什么eccDNADNA.淋巴结转移是淋巴结的转移.分子癌症生物学分子生物学

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