离子通道TRPV5调节B细胞信号和激活
Trisha Mahtani1, Hena Sheth1, L K Smith2
1Department of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada.
Frontiers in immunology
|May 2, 2024
概括
暂时受体潜在化物5 (TRPV5) 通道对于B细胞受体信号和激活至关重要. TRPV5淘汰赛小鼠显示B细胞扩散,收缩和T-依赖抗原反应受损.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 离子通道生理学 离子通道生理学
背景情况:
- B细胞激活涉及通过储存运行的入 (SOCE) 和释放激活 (CRAC) 道的流.
- 特定于B细胞的SOCE淘汰并没有显著影响幽默免疫力,这表明涉及其他通道.
研究的目的:
- 为了研究透过渡受体潜能 (TRP) 通道TRPV5在B细胞反应中的作用.
- 为了生成和分析TRPV5淘汰赛 (KO) 小鼠,以了解其在B细胞信号传递中的功能.
主要方法:
- 定量聚合酶连锁反应 (qPCR) 屏幕用于识别B细胞中的候选通道.
- 通过CRISPR-Cas9基因编辑生成TRPV5淘汰赛小鼠.
- 在TRPV5KO小鼠中分析B细胞发育,流入,B细胞受体 (BCR) 信号发送,细胞扩散和T-依赖抗原反应.
主要成果:
- TRPV5被确定为B细胞表达的候选通道,并且在刺激时被发现会极化为BCR集群.
- TRPV5 KO小鼠表现出正常的B细胞发育和数量,在BCR刺激后流量没有受损.
- TRPV5 KO B细胞显示扩散和收缩受损,BCR信号减少 (酸铁水平),免疫后早期T-依赖抗原特异性反应减少.
结论:
- TRPV5在B细胞受体信号传递,B细胞激活和免疫反应中发挥着重要作用.
- 在TRPV5缺乏的B细胞中可能存在涉及其他通道 (如ORAI1) 的补偿机制.
更多相关视频
08:27Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Published on: January 7, 2019
9.5K
16:10A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
Published on: March 22, 2012
23.9K
相关概念视频
Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
B Cell Activation and Differentiation
1.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
1.7K
Mechanically-gated Ion Channels
6.4K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
6.4K
T Cell Types and Functions
1.0K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.0K
Signal Transduction: Overview
8.5K
Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...
Typically, signal transduction involves three...
8.5K
Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
