屏幕印刷石墨烯的生物层-干扰仪引导功能化,用于无标签的电化学病毒检测
Beata M Szydlowska1, Cícero C Pola2, Zizhen Cai1
1Department of Materials Science and Engineering, Northwestern University, Evanston, Illinois 60208, United States.
ACS applied materials & interfaces
|May 2, 2024
概括
这项研究介绍了一种新的屏幕打印石墨烯生物传感器,用于快速和敏感的电化学检测SARS-CoV-2变种. 该平台在临床样本上表现出高的特异性和性能,为护理点诊断铺平了道路.
科学领域:
- 材料科学 材料科学 材料科学
- 生物传感器技术技术
- 分析化学 分析化学
背景情况:
- 增材制造,特别是幕印刷,为低成本,高通量生物传感器生产提供了潜力.
- 幕印刷需要优化的油墨,后处理和功能化,以实现高性能生物传感.
- 开发针对SARS-CoV-2等各种病原体的敏感和特定的生物传感器对于公共卫生至关重要.
研究的目的:
- 开发一个基于石墨烯电极的屏幕打印生物传感平台,用于敏感和特定的SARS-CoV-2电化学检测.
- 利用生物层干涉测量 (BLI) 选择最佳抗体,对抗多种SARS-CoV-2变种.
- 为了证明平台的快速检测和分析临床样本的能力.
主要方法:
- 石墨烯电极的丝网打印,然后进行热,以提高表面积和导电性.
- 生物层干涉测量 (BLI) 用于无标签查和对SARS-CoV-2变种 (三角形,欧米克龙,野生型) 具有广泛特异性的抗体的选择.
- 电化学阻抗光谱 (EIS) 用于通过对功能化石墨烯电极的抗体-抗原结合检测SARS-CoV-2.
主要成果:
- 优化的丝网打印石墨烯电极表现出高表面积和电导率.
- BLI成功地确定了对多个SARS-CoV-2变体的高亲和力和对H1N1流感的低亲和力抗体.
- 由此产生的电化学免疫传感器实现了快速检测 (55秒读取),低检测极限 (Omicron 500 ag/mL) 和高选择性.
- 生物传感器在临床唾液样本中成功检测到SARS-CoV-2 Omicron,其度低至10^3副本/毫升.
结论:
- 屏幕打印的石墨烯电极,当与BLI选择的抗体相结合时,形成一个高性能生物传感平台.
- 这种方法可以快速,灵敏和特定地检测多个SARS-CoV-2变种.
- 这种通用方法对开发各种护理点免疫传感器用于病原体检测具有前景.
更多相关视频
07:51Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
Published on: February 1, 2022
3.2K
09:39Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology
Published on: March 31, 2022
3.3K
相关概念视频
03:31Labeling DNA Probes
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
01:24Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...