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CsSHMT3基因在盐应激下增强黄瓜幼苗的生长和发育

Zhuohui Zhang¹, Xuemei Hou¹, Rong Gao¹

¹College of Horticulture, Gansu Agricultural University, 730070, Lanzhou, PR China.

Plant molecular biology

|May 2, 2024

概括

血清基甲基转移酶3 (SHMT3) 基因增强了黄瓜幼苗的盐耐受性. 过度表达CsSHMT3会改善生长和应激反应,而沉默会降低耐受性.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
压力生理学压力生理学

背景情况:

在全球范围内,盐应激显著限制了作物生产率.
血清基甲基转移酶 (SHMT) 基因对于植物生长和应激反应至关重要.
在植物盐耐受性中SHMT3的特定作用仍然在很大程度上未被探索.

研究的目的:

为了研究黄瓜 (Cucumis sativus) 盐耐受性中CsSHMT3基因的功能.
确定CsSHMT3基因过度表达和沉默对黄瓜幼苗对盐应激反应的影响.

主要方法:

在黄瓜幼苗中对CsSHMT3表达 (过度表达和沉默) 的基因操纵.
在盐应力下评估生理参数,包括叶绿素含量,光合作用率,素含量,抗氧化酶活性和膜损伤标志物 (MDA,H2O2,O2·−,相对导电性).
分析关键与压力相关的基因 (SOD,CAT,SOS1,SOS2,NHX,HKT) 的表达水平.

主要成果:

过度表达CsSHMT3显著增加了叶绿素,光合作用率,林含量和抗氧化酶活性,同时降低了MDA,H2O2,O2·−和盐应力下的相对导电性.
沉默CsSHMT3导致减少叶绿素,光合作用率,プロ林含量和抗氧化酶活性,同时增加MDA,H2O2,O2·−和盐应激下的相对导电性.
过度表达CsSHMT3调高了应激反应基因 (SOD,CAT,SOS1,SOS2,NHX,HKT),而沉默则调低了它们.

结论:

CsSHMT3基因积极调节黄瓜幼苗的盐应激耐受性.
CsSHMT3可能通过调节抗氧化活性,透调整和光合作用来提高盐分耐受性.
该基因在调节关键的透性,光合作用,抗氧化和与压力相关的基因的表达方面发挥着作用.

关键词:

抗氧化剂活性抗氧化剂活性奥斯莫斯调整的调整过度表达是一种过度表达.光合作用光合作用有反应性氧物种的反应性氧物种.病毒诱导的基因沉默是病毒引起的.

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