使用DArT-Seq技术对来自三个不同的农业生态区的商业杂交和土著之间的遗传多样性的表型特征和分析

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Kwaku Adomako¹, Selorm Sovi^1,2, Bismark Kyei^1,3

¹Department of Animal Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

|May 2, 2024

概括

用SNP标记物分析了加纳的遗传多样性. 商业杂交品种的多样性高于本土生态型,这表明当地品种的遗传改进潜力.

科学领域:

动物遗传学动物遗传学
人口基因组学人口基因组学
农业科学农业科学

背景情况:

原住民对各种环境具有独特的适应性,这些适应性是由选择压力所塑造的.
人工选择和环境因素推动了种群的全基因组分歧.
了解遗传多样性对于保护和繁殖计划至关重要.

研究的目的:

确定来自加纳农业生态区 (AEZ) 和商业杂交的土著的基因组多样性和种群结构.
评估土著生态型和商业杂交品种之间的遗传差异化.
根据观察到的多样性,探索基因改进的潜力.

主要方法:

使用DArT-Seq技术在82只 (62只本土,26只商业) 上生成了SNP标记.
计算了多态性信息内容 (PIC) 值和异构性.
人口结构使用主要组件分析 (PCA) 和双对FST值进行了分析.

主要成果:

总共分析了58,353个SNP标记,其中PIC值从0.0到0.5.5不等.
商业交叉品种 (CC) 呈现的遗传多样性 (预期异构性=0.220) 比本土鸟类更高.
PCA发现CC,SDR和GS聚集在一起,与商业杂交不同,表明它们形成了一个单一的种群.

结论:

来自加纳SDR,CS和GSAEZ的土著可以被认为是一个单一的种群.
SDR 的生长表现更好,可能是由于农业副产品的可用性.
当地生态型中的遗传多样性为未来的遗传改进策略提供了基础.

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