德拉蛋白招募介质复合体子单元MED15,以协同激活陆地植物中的转录
Jorge Hernández-García1,2, Antonio Serrano-Mislata1, María Lozano-Quiles1
1Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, Valencia 46022, Spain.
概括
德拉蛋白招募中介复合体以促进转录,这是陆地植物中保存的机制. 这揭示了DELLA如何作为协作激活剂,影响植物的发育和进化.
科学领域:
- 植物分子生物学 植物分子生物学
- 转录条例 转录条例 转录条例
- 进化生物学 进化生物学
背景情况:
- 德拉蛋白质是植物中吉伯雷林信号传递的关键负调节者.
- 它们在隔离转录因子 (TFs) 中的作用已知,但它们的协激活器功能尚不清楚.
研究的目的:
- 阐明DELLA蛋白质作为转录协激活剂的机制.
- 研究陆地植物中这种同活化机制的保护.
主要方法:
- 使用分子生物学技术研究了Arabidopsis中的DELLA-Mediator复杂相互作用.
- 检查了MED15在DELLA中介协作激活中的作用.
- 研究了肝草 (Marchantia polymorpha) 中的DELLA-MpMED15相互作用.
主要成果:
- 德拉蛋白直接将中介复合物招募到阿拉比多普西斯的特定DNA位置,从而增强转录.
- 这种相互作用涉及DELLA N终端域和MED15 KIX域.
- 丧失MED15功能会影响与细胞因素和吉伯雷林相关的过程,突出显示DELLA协激活.
- 在Marchantia polymorpha中DELLA蛋白也招募了MpMED15,证明了协同激活.
结论:
- 德拉蛋白通过招募介质复合体,作为转录协活性剂.
- 这种机制在 angiosperms (Arabidopsis) 和 bryophytes (Marchantia polymorpha) 之间得到保护.
- 德拉的中介体依赖性联合激活是一种古老的机制,可以追溯到陆地植物的最后一个共同祖先.
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