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通过RNA-seq分析,探索和验证ADAR1删除诱导的NAFLD中的铁亡相关基因

Xuecui Yin¹, Yang Mi², Xiaohan Wang³

¹Henan Key Laboratory of Helicobacter Pylori, Microbiota and Gastrointestinal Cancer, Marshall Medical Research Center, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China; Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China; Department of Gastroenterology, the Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan, China.

International immunopharmacology

|May 2, 2024

概括

作用于RNA 1 (ADAR1) 下调的腺胺酶与非酒精性脂肪性肝病 (NAFLD) 和铁亡有关. 本研究确定了NAFLD预防和治疗策略的关键生物标志物.

科学领域:

肝病学肝病学是一种肝病学.
分子生物学分子生物学
生物化学生化学

背景情况:

铁,由铁和脂质过氧化物积累驱动,有助于非酒精性脂肪性肝病 (NAFLD).
作用于RNA 1 (ADAR1),参与脂质代谢和免疫调节的腺氨酸脱氨酶在NAFLD通过铁死病变的发病过程中的作用尚不清楚.

研究的目的:

调查ADAR1在与铁死相关的NAFLD中的作用.
为了确定NAFLD的潜在生物标志物和治疗目标.

主要方法:

在NAFLD患者中分析了ADAR1表达 (GSE66676数据库).
检查了ADAR1对NAFLD细胞中铁亡标记物的抑制作用 (体外/体内).
利用RNA-seq,Venn图,GO,KEGG和PPI网络分析来识别关键的基因.

主要成果:

在NAFLD患者中,ADAR1表达的下调.
ADAR1敲击改变了ferroptosis标记物,并差异地表达了22个与ferroptosis相关的基因.
鉴定出NOS2,PTGS2,NOX4,ALB,IL6和CCL5是与中央铁死相关的基因.

结论:

通过铁亡信号传递,ADAR1的删除与NAFLD的病原发生有关.
NOS2,PTGS2,ALB和IL6显示出与ADAR1删除相关的NAFLD生物标志物的潜力.
这些发现表明NAFLD的新诊断和治疗策略.

关键词:

在 ADAR1 中,ADAR1 是铁化是铁化的一种.非酒精性脂肪性肝病是非酒精性脂肪性肝病.免疫学免疫学免疫学肝脏疾病是一种肝脏疾病.

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