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Updated: Apr 12, 2026

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
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高活性重组人猪尿酶突变蛋白的合理化设计,可溶性表达和PEG修饰

Mingjie Tong1, Shengli Wang2, Junxi Luan1

  • 1Institute of Biomedicine and National Engineering Research Center of Genetic Medicine, College of Life Science and Technology, Jinan University, Guangzhou 510632, People's Republic of China.

International journal of biological macromolecules
|May 2, 2024
PubMed
概括

研究人员设计了一种新型的尿酶,通过增强尿酸代谢来治疗高尿血症. 这种经过修改的酶,尿-10,显示出高活性和稳定性,为痛风和高尿酸水平提供了有前途的治疗候选者.

关键词:
生物活性 生物活性同性学 同性学是指同性学.人类猪尿的基梅拉.

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Production of Pharmaceuticals01:30

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
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