相关实验视频

Updated: Jun 27, 2025

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

通过使用双重质量标签标记的定量蛋白质组学分析,识别涉及血栓血管炎消灭症发展的S100A8/A9

Jing Chen¹, Chunfang Chen², Lili Wang²

¹Guangzhou Institute of Cardiovascular Disease, Guangdong Key Laboratory of Vascular Diseases, State Key Laboratory of Respiratory Disease, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China.

Cellular signalling

|May 2, 2024

概括

阻塞性血栓血管炎 (TAO) 涉及动脉炎症和阻塞. 在TAO中确定了早期的S100A8/A9激活,这表明它是TAO预防和治疗的潜在治疗标.

科学领域:

血管生物学血管生物学
蛋白质组学是指蛋白质组学.
药理学药理学是指药理学的学科.

背景情况:

阻塞性血栓血管炎 (TAO) 是一种血管疾病,导致远端动脉炎症和阻塞.
目前对TAO的药物治疗和手术干预是有限的.
人们对TAO的确切病原性尚不了解.

研究的目的:

通过定量蛋白质组学来确定涉及TAO发展的关键蛋白质和途径.
调查TAO中针对S100A8/A9信号的治疗潜力.

主要方法:

使用双重质量标签 (TMT) 的正常和TAO大鼠模型的比较蛋白质组分析.
生物信息学工具用于差异性蛋白质表达和途径分析.
在TAO模型中对S100A8/A9抑制剂帕基尼莫德的体外和体内评估.

主要成果:

在TAO大鼠中鉴定出1385种差异表达蛋白 (DEP),主要涉及细胞粘附,迁移和细胞骨调节.
涉及的关键途径包括actin细胞骨调节,血管光滑肌收缩和焦点粘附.
帕基尼莫德治疗减少了光滑肌肉细胞的增殖和迁移,促进了表型切换,并缓解了TAO大鼠的血管狭窄.

结论:

在大腿动脉中S100A8/A9的早期激活在TAO病变发生中起着重要作用.
针对S100A8/A9信号提供了一个有前途的新型治疗策略,用于TAO的预防和治疗.

关键词:

在Paquinimod中使用了Paquinimod.蛋白质组学是指蛋白质组学.S100A8 / A9 / A9 / S100A8 / A9 / S100A8 / A9 / S100A8 / A9 / S100A9 / S100A8 / S100A9 / S100A8 / S100A9 / S100A9 / S100A8 / S100A9 / S100A9 / S100A9 / S100A8 / S100A9 / S100A9 / S100A8 / S100A9 / S100A9 / S100A8 / S100A9 / S100A8 / S100A9 / S100A9 / S100A8 / S100A9 / S100A8 / S100A9 / S100A8 / S100A9 / S100A8 / S100A9 / S100A9 / S100A8 / S100A9 / S100A8 / S100A9 / S100A8 / S100A8 / S100A8 / S100A8 / S100A9 顺滑肌肉细胞是一种平滑肌肉细胞.血栓血管炎的消灭者

更多相关视频

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples

Published on: November 13, 2021

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...