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通过隔离的有氧脱应变在垂直流构建湿地中增强去除

Hongxu Zhu¹, Yanmei Liu², Zhenghua Peng³

¹Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China; Kunming Metallurgical Research Institute Co., Ltd., Kunming, 650031, China.

|May 2, 2024

概括

添加有氧脱剂RC-15显著改善了建筑湿地中的去除. 这种细菌剂增强了关键的脱基因和微生物多样性,显示了未来废水处理应用的潜力.

科学领域:

环境微生物学环境微生物学
废水处理工程废水处理工程
湿地生物地球化学湿地生物地球化学

背景情况:

从废水中去除气对于环境保护至关重要.
建筑湿地 (CWs) 在废水处理方面是有效的.
细菌剂可以提高CW中的去除效率.

研究的目的:

调查有氧脱剂 (RC-15) 对垂直流构造湿地 (CW) 中的去除的影响.
分析不同CW深度的功能性脱基因的丰度和分布.
描述微生物群落结构及其在增强脱化中的作用.

主要方法:

将细菌剂RC-15添加到一个垂直流构造的湿地中.
定量PCR (qPCR) 用于定量化脱基因 (nirS,napA,nirK).
16S rRNA基因测序用于不同CW深度的微生物社区分析.

主要成果:

与对照组相比,RC-15显著增加了酸盐 (NO3-) 和总 (TN) 的去除.
鉴定出nirS基因是一个主要的脱基因.
RC-15增强了napA和nirK基因的丰富性,具有明显的深度分布.
细菌添加导致了更多样化的脱路径和增加了16S.rRNA的丰富性.
蛋白质细菌和Chloroflexi是占主导地位的类,而Thauera,Aeromonas和Ardenticatenales表现出更高的丰度.

结论:

有氧脱剂RC-15有效地提高了建筑湿地中的去除.
特定的脱基因 (napA,nirK) 和微生物属 (Thauera,Aeromonas,Ardenticatenales) 在这个过程中发挥关键作用.
这些发现支持使用细菌剂来优化废水处理中的去除.

关键词:

有氧脱剂是一种有氧脱剂.建造的湿地建造的湿地欧洲化是什么意思?功能性基因是指功能性的基因.

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