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Updated: Jun 27, 2025

Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses
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Nanogold Labeling of the Yeast Endosomal System for Ultrastructural Analyses

Published on: July 14, 2014

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一种先进的快速方法,用于评估多种免疫标记,使用基于低能量STEM的金纳米粒子

František Kitzberger1,2, Shun-Min Yang3,4, Jiří Týč5

  • 1Laboratory of Electron Microscopy, Institute of Parasitology, Biology Centre CAS, 370 05, České Budějovice, Czech Republic. Frantisek.kitzberger@paru.cas.cz.

Scientific reports
|May 2, 2024
PubMed
概括

这项研究引入了一种使用高分辨率扫描电子显微镜 (HRSEM) 的新方法,用于先进的免疫标记应用,同时检测超薄截面两侧的金纳米粒子 (Au NPs).

关键词:
背散电子成像技术 背散电子成像技术高分辨率扫描电子显微镜 (HRSEM) 的使用免疫标记的使用蒙特卡洛模拟的蒙特卡洛模拟.扫描传输电子显微镜扫描传输电子显微镜同时检测多个免疫黄金标记物.

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相关概念视频

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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