新的假定因果突变与Nellore牛的脂肪特征相关,这些突变是由位于开放色素区域的eQTL发现的
Ingrid Soares Garcia1, Bárbara Silva-Vignato1, Aline Silva Mello Cesar2
1Department of Animal Science, College of Agriculture "Luiz de Queiroz", University of São Paulo, Piracicaba, SP, Brazil.
Scientific reports
|May 2, 2024
概括
了解肌肉内脂肪 (IMF) 和背部脂肪厚度 (BFT) 的遗传变异是肉质的关键. 这项研究整合了基因组和转录组数据,以确定影响这些经济重要特征的监管变异.
科学领域:
- 动物遗传学动物遗传学
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 肌肉内脂肪 (IMF) 和背部脂肪厚度 (BFT) 是影响肉质的关键经济特征.
- 对IMF和BFT的遗传控制需要进一步调查,以改善畜牧养殖.
研究的目的:
- 识别遗传变异并了解它们在IMF和BFT中的监管作用.
- 整合基因组和转录基因组数据,对脂肪沉积特征进行全面分析.
主要方法:
- 通过整合RNA-seq和基因组数据,生成了553,581个SNP的变异面板.
- 进行表达量的特征位点 (eQTL) 分析以识别cis-和trans-eQTL.
- 使用ATAC-Seq将eQTL映射到染色体可访问区域.
主要成果:
- 已经确定了36916个cis-eQTL和14408个trans-eQTL.
- 发现了27个与BFT (3) 和IMF (24) 有关的eQTL.
- 发现了与脂质代谢,免疫反应和细胞骨重塑通路相关的eQTLs,其中6个位于调节区域.
结论:
- 提供了对IMF和BFT转录监管的新见解.
- 发现了影响脂肪沉积特征的假定调节变异.
- 这项研究有助于理解肉质特征的遗传基础.
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