赛纳普托塔格明-11促进了在高尔吉后载荷囊中的前突触信号复合体的组装
Luca Trovò1, Stylianos Kouvaros1, Jochen Schwenk2
1Department of Biomedicine, University of Basel, Basel, Switzerland.
EMBO reports
|May 2, 2024
概括
突触胺-11 (Syt11) 作为囊泡支架,在囊泡内组装GABAB受体 (GBR) 和Cav2.2通道信号复合体. Syt11确保稳定的GBR/Cav2.2在突触中的表达,这对神经传递至关重要.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- GABAB受体 (GBRs) 是与G蛋白合的受体,调节大脑突触传输.
- GBRs关键门Cav2.2-type Ca2+通道,但这些复杂的组装地点是未知的.
研究的目的:
- 为了确定稳定的GBR/Cav2.2信号复合体形成的细胞区.
- 阐明Synaptotagmin-11 (Syt11) 在GBR/Cav2.2复杂组合和局部化中的作用.
主要方法:
- 使用生物化学测试研究了蛋白质相互作用.
- 检查了GBRs和Cav2.2通道的细胞定位和贩运.
- 在Syt11淘汰赛小鼠中分析了神经元功能.
主要成果:
- Syt11与KCTD16 (一个GBR子单元) 和Cav2.2通道结合.
- Syt11将GBR和Cav2.2通道招募到Golgi后囊泡中进行复杂的组装.
- 通过抑制内部化,Syt11稳定了血膜上的GBRs和Cav2.2通道.
- Syt11淘汰赛神经元显示了降低的前突触GBRs,Cav2.2通道,神经递质释放和GBR介导的抑制.
结论:
- Syt11作为囊泡支架蛋白质起作用,对于组装GBR/Cav2.2信号复合体至关重要.
- 这种机制有助于预先组装的功能信号单元插入到突触膜中.
- Syt11对于神经元中GBR/Cav2.2复合物的稳定表达和功能至关重要.
相关概念视频
Fusion of Secretory Vesicles with the Plasma Membrane
11.1K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
11.1K
SNAREs and Membrane Fusion
10.9K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10.9K
Vesicular Tubular Clusters
2.5K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.5K
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K
Assembly of Signaling Complexes
5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Protein Translocation Machinery on the ER Membrane
4.6K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
4.6K


