在 Dioscorea bulbifera Linn. 的抗菌活性. 提取物及其活性成分flavanthrinin对抗与皮肤有关的细菌
Donruedee Sanguansermsri1,2, Phanchana Sanguansermsri3, Kittisak Buaban4
1Department of Microbiology and Parasitology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.
BMC complementary medicine and therapies
|May 2, 2024
概括
迪奥斯科雷亚球提取物及其化合物黄氨酸显示强烈的抗菌活性对抗Staphylococci,包括MRSA. 这种天然化合物为治疗皮肤感染提供了一种安全的,互补的方法.
科学领域:
- 植物化学 植物化学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 这种植物是 Dioscorea bulbifera Linn. (空气土豆) 在泰国传统上用于伤口护理.
- 需要对其抗菌性质和活性化合物的科学评估.
- 这项研究的重点是D. bulbifera对皮肤病原体的抗菌功效.
研究的目的:
- 研究D. bulbifera提取物对与皮肤相关的细菌的抗菌活性.
- 从D. bulbifera中分离和描述活性抗菌剂.
- 评估提取物及其活性化合物的细胞毒性.
主要方法:
- 使用各种溶剂提取D. bulbifera bulbils的方法.
- 通过微稀释评估抗菌活性,并确定最小抑制度 (MIC).
- 使用薄层染色学 (TLC) 生物自拍,列染色学和核磁共振 (NMR) 光谱学识别和净化活性化合物.
- 使用Vero细胞进行细胞毒性评估.
主要成果:
- 乙酸乙烯提取物显示出最显著的抗菌活性.
- D. bulbifera提取物对皮肤细菌的MIC为0.78-1.56毫克/毫升.
- 隔离的化合物flavanthrinin表现出增强的活性,MIC为0.02-0.78 mg/mL,细胞毒性低 (CC50 = 0.41 ± 0.03 mg/mL).
- 提取物和黄素都对金黄色葡萄球菌,MRSA和S. epidermidis有效.
结论:
- 迪奥斯科里亚黄菌提取物及其成分黄素具有显著的抗菌活性,可以对抗葡萄球菌,包括耐甲基西林黄金葡萄球菌 (MRSA).
- 由于其强大的抗菌作用和低细胞毒性,弗拉万宁显示出作为皮肤感染的安全,补充性治疗剂的潜力.
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