资本细胞被排除在外:可能的进化转变导致左侧组织的损失,而不是由主导负BMPR1增加的神经组织
Nicole B Webster1,2, Néva P Meyer3
1Biology Department, Clark University, 950 Main Street, Worcester, MA, 01610, USA.
Neural development
|May 2, 2024
概括
BMP信号影响神经系统的发育不同于以前认为的类动物. 这项研究揭示了一种新的不对称组织损失表型,挑战了BMP在双胞胎中中枢神经系统 (CNS) 形成中的保留作用.
科学领域:
- 进化发育生物学 进化发育生物学
- 进行比较的基因组学.
- 神经科学是一个神经科学.
背景情况:
- 了解双胞胎细胞中中央神经系统 (CNS) 的进化起源和遗传同质性至关重要.
- 假设骨形态遗传蛋白 (BMP) 信号通路在建立背腹 (D-V) 轴和限制神经特征方面发挥着保留的作用.
- 在多种类型中研究BMP信号对于全面的进化视角至关重要.
研究的目的:
- 为了研究BMP信号的作用在Capitella teleta的合体的发展,一个代表的Spiralia.
- 为了确定BMP信号是否在确定D-V轴和中枢神经系统时得到保留,在这个类物种中.
主要方法:
- 利用CRISPR/Cas9基因编辑来击倒BMP抗剂Chordin-like (Ct-Chordin-like) 的作用.
- 在Capitella teleta表达了一个主导负的BMP受体1 (Ct-BMPR1).
- 分析了发育现象型,包括组织形成和D-V轴模式.
主要成果:
- 主导负的Ct-BMPR1表达没有改变D-V轴形成,但导致左侧组织 (眼睛,大脑,前肠,中皮) 的独特不对称损失.
- 宫外BMP4添加逆转了表型,导致右侧组织损失.
- 克里斯普尔对Ct-Chordin-like的破坏导致左侧非对称的组织损失,主要是在干部.
结论:
- 在D-V轴和中枢神经系统建立中BMP信号的作用在Pleistoannelida (和潜在的Spiralia) 与其他双边生物相比不同.
- 在Capitella teleta.中,BMP信号对神经系统的界限没有必要.
- 揭示了一个新的不对称的发育表型,突出了BMP信号在进化中的复杂性.
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