Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Nanotechnology in reproductive medicine: from gamete engineering to precision therapeutics.

RSC advances·2026
Same author

A Single-Enzyme Activated CRISPR-Cas12a Nano System via Subtly Balanced dsDNA for Kinetic-Gated UDG Detection and Spatiotemporal Cellular Imaging.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Deep convolutional and fully-connected DNA neural networks.

Nature communications·2025
Same author

An Endo IV-driven signal amplifier for sensitive detection of cancer biomarker TdT.

The Analyst·2025
Same author

Uncovering the Fluctuation of Peroxynitrite during Early Embryonic Development Using an Integrative Nanobeacon.

Analytical chemistry·2025
Same author

Nicking enzyme assisted amplification combined with CRISPR-Cas12a system for one-pot sensitive detection of APE1.

The Analyst·2025

相关实验视频

Updated: Jun 27, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.7K

基于可配置逻辑块的可擦除和现场可编程DNA电路.

Yizhou Liu1,2, Yuxuan Zhai1, Hao Hu2

  • 1School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, 430023, China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|May 3, 2024
PubMed
概括

这项研究引入了一种可擦除,可现场编程的DNA电路,其灵感来自可配置逻辑块 (CLB). 这项创新允许各种逻辑操作和计算,显著提高DNA电路的实用性.

关键词:
DNA 逻辑电路的逻辑回路.DNA纳米技术 DNA纳米技术剪片介导的链位移反应反应.可配置逻辑区块的区块.现场编程 现场编程

更多相关视频

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.4K

相关实验视频

Last Updated: Jun 27, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

1.7K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.2K
Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
07:50

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

14.4K

科学领域:

  • 生物技术是生物技术.
  • 分子工程分子工程分子工程
  • 合成生物学 合成生物学

背景情况:

  • DNA的生物相容性和可编程性使其成为人工逻辑网络的理想选择.
  • 当前的DNA逻辑电路通常是一次性,由于缺乏现场可编程性,限制了它们的实际应用.

研究的目的:

  • 使用可配置逻辑块 (CLB) 架构开发一个可擦除和现场可编程的DNA电路.
  • 为了证明这种新的DNA电路设计的多功能性和实用性,用于复杂的计算.

主要方法:

  • 设计和制造基于CLB的可删除的现场可编程DNA电路.
  • 采用剪贴线条作为动态电路重新配置的操作控制信号.
  • 演示了基本的逻辑门 (OR,AND) 和复杂的二进制计算器 (半子,半减法).

主要成果:

  • 成功证明了基于CLB的DNA逻辑门的可擦除性和现场可编程性.
  • 在双层电路上实现多轮编程,用于各种逻辑操作.
  • 实现了基本的二进制计算器,模拟了基于的电路功能,以及一个具有七个操作的综合电路.

结论:

  • 基于CLB的可删除的现场可编程DNA电路显著提高了DNA逻辑网络的可行性.
  • 这种设计提供了效率和便利性,为在先进的DNA计算应用中广泛使用铺平了道路.