在正在使用的机械流体中流通的无细胞菌蛋白占主导地位及其抗原性潜力
Harish Chandra1, Bethany Ahlers2, Ying Wai Lam2
1Pulmonary/Microbial Pathogenesis and Immunotoxicology Laboratory, Department of Environmental and Public Health Sciences, University of Cincinnati College of Medicine, Cincinnati, OH45267-0056, USA.
受到Mycobacterium immunogenum (MI) 污染的金属加工液 (MWF) 的职业暴露可能导致肺部疾病. 这项研究确定了MWF中占主导地位的MI蛋白和抗原,以帮助早期检测和评估过敏性肺炎 (HP) 的风险.
科学领域:
- 职业健康和安全 职业健康和安全
- 免疫学和传染病.
- 蛋白质组学和生物信息学
背景情况:
- 职业暴露于由*Mycobacterium immunogenum* (MI) 殖民的金属加工液 (MWF) 与机械工人过敏性肺炎 (HP) 有关.
- 在MWF中早期检测MI蛋白质,特别是抗原蛋白质,对于监测和预防HP至关重要.
研究的目的:
- 直接从现场采集的,正在使用的MWF中识别主导MI蛋白和抗原.
- 开发一种综合性免疫蛋白质-免疫信息学方法,用于检测MWF中的MI组件.
主要方法:
- 基于DNA的查发现了MI阳性MWF,随后进行了菌株隔离和培养.
- 综合性免疫蛋白组学 (1D/2D凝电泳,LC-MS/MS) 和免疫信息学被用来分析无细胞MI蛋白和抗原.
- 与抗MI血清 (子和患者) 的血清活性指导蛋白质识别.
主要成果:
- 一个新的MI菌株 (MJY-27) 被确定.
- 检测到86个MI蛋白,其中29个显示出显著的丰度.
- 对T细胞表位分析确定了13种具有高结合频率的HLA-I和II等位基因的蛋白质,而抗原性分析显示每种蛋白质有6到37个抗原决定因素. 一个候选者是经过验证的强大的B细胞和T细胞抗原.
结论:
- 这项研究首次报告了在现场使用的MWF中*M. immunogenum*的主导蛋白质和假定抗原.
- 这项工作代表了开发MWF监测策略的重大进展,用于机械加工环境中的暴露和HP风险评估.
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