psilocybin,迷幻提取物和5-hydroxytryptophan对大脑直接早期基因表达的作用:与神经受体调节器的相互作用
Elad Lerer1,2, Alexander Botvinnik1, Orr Shahar1
1Biological Psychiatry Laboratory and Hadassah BrainLabs Center for Psychedelic Research, Hadassah Medical Center, Hebrew University, Jerusalem, Israel.
Frontiers in pharmacology
|May 3, 2024
概括
псилоцибин (PSIL) 和 псилоцибин提取物 (PME) 在小鼠大脑中显著增加了即时早期基因表达 (cfos,egr1). 5-基 (5-HTP) 没有影响这些基因,这表明cfos和egr1表达和迷幻效应之间存在联系.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 直接早期基因 (IEG) 对于神经可塑性等细胞过程至关重要.
- псилоцибин (PSIL), псилоцибин提取物 (PME) 和5-基酸 (5-HTP) 是具有精神活性或前体特性的化合物.
- 了解IEG调节是破译迷幻作用分子机制的关键.
研究的目的:
- 研究PSIL,PME和5-HTP对小鼠体感皮层 (SSC) 中IEGs (cfos,egr1,egr2) 的表达的影响.
- 探索IEG诱导和迷幻效应之间的关系,例如头部抽反应 (HTR).
- 为了检查血清受体调节对PSIL诱导的IEG表达的影响.
主要方法:
- 雄性C57Bl/6j小鼠接受了PSIL或5-HTP,有或没有血清受体调节剂.
- 实时定量PCR (qPCR) 用于测量IEG mRNA表达的一小时后.
- 一项复制研究包括使用 PME 的治疗.
主要成果:
- 在SSC中,PSIL显著上调了cfos,egr1和egr2mRNA水平.
- 5-HTP没有显著改变IEG表达.
- 在复制研究中,PSIL和 PME对cfos和egr1进行了上调,但不对egr2进行上调.
- 血清受体调节剂通常不会影响PSIL诱导的IEG表达,除了增强egr2上调的5-HT2C抗剂.
结论:
- PSIL和 PME,但不是5-HTP,在小鼠SSC中显著增加cfos和egr1表达.
- egr1和cfos的表达可能是与含有psilocybin的物质的迷幻效应相关的分子标记物.
- 这些发现有助于理解迷幻药物的神经生物学基础.
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