细菌细胞杀伤性扩张毒素和SARS-CoV-2病毒使用细胞氨酸 (synaptogyrin-2) 依赖途径进入细胞
Kathleen Boesze-Battaglia1, Gary H Cohen1, Paul F Bates2
1Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Frontiers in cellular and infection microbiology
|May 3, 2024
概括
纤维氨酸是微微囊中的蛋白质,对于细菌毒素和SARS-CoV-2进入宿主细胞至关重要. 破坏细胞氨酸会使细胞对这些病原体产生抵抗力,这表明有一个共同的进入途径.
科学领域:
- 分子生物学分子生物学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 聚合菌actinomycetemcomitans细胞杀伤性扩张毒素 (Cdt) 通过通过脂质内部化其子单元来毒害各种宿主细胞.
- 蜂氨酸对于Cdt子单元在突触类微粒细胞 (SLMVCg+) 中的贩运和Cdt诱导的毒性至关重要.
- SARS-CoV-2的进入还涉及富含胆固醇的脂质和ACE-2受体,这表明宿主细胞入侵机制的潜在相似性.
研究的目的:
- 调查塞卢吉林在Cdt和SARS-CoV-2内部化途径中的作用.
- 确定细胞氨酸是否是CDT和SARS-CoV-2的进入机制中的关键组成部分.
- 为了确定CDT子单元,SARS-CoV-2尖端蛋白和细胞氨酸之间的特定结合相互作用.
主要方法:
- 使用lentiviral shRNA制备细胞氨酸缺乏的Calu-3细胞 (Calu-3Cg-) 的方法.
- 对VSV/SARS-CoV-2-spike和VSV/VSV-G伪型病毒感染的细胞耐药性的评估.
- 对合成纤维素和Cdt子单元或SARS-CoV-2尖端蛋白之间的结合相互作用的表面等离子体共振分析.
主要成果:
- 纤维素缺乏细胞对VSV/SARS-CoV-2-spike伪型病毒表现出耐药性,对VSV/VSV-G伪型病毒表现出部分耐药性.
- 在SLMVCg+中,Cdt子单元与细胞氨酸的中间外环 (MOL) 结合.
- SARS-CoV-2 全长尖蛋白及其 S1 域与细胞原蛋白的不同区域结合 (分别为内环 1A 和 N-终端).
结论:
- 塞卢吉林在Cdt和SARS-CoV-2进入宿主细胞方面发挥着关键作用.
- 含有细胞氨酸 (SLMVCg+) 的突触类微片代表了病原体和毒素进入的潜在常见途径.
- 病原体成分和细胞氨酸之间的特定分子相互作用突出显示了宿主细胞入侵的保存机制.
关键词:
聚合菌是Actinomycetemcomitans的组合体之一.这就是SARS-CoV-2病毒.单元格的入口是细胞的入口.细胞氨酸 (cellugyrin) 是一种氨酸.细胞杀伤性扩张毒素病原体是一种病原体.类似于突触的微微环.这种药物是synaptogyrin-2更多相关视频
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