相关实验视频

Updated: May 2, 2026

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Published on: May 7, 2018

高通量单细胞质谱法通过分解脂C[双键,长度为m-dash]C异构体,使代谢网络分析成为可能

Simin Cheng¹, Chenxi Cao², Yao Qian²

¹Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology Bejing 100029 China daixh@nim.ac.cn.

Chemical science

|May 3, 2024

概括

这项研究引入了一种高通量单细胞质谱平台,用于详细的脂质分析,使精确的结构特征和同位素量化能够改善细胞表型和途径研究.

科学领域:

分析化学分析化学
生物化学生物化学
细胞生物学细胞生物学

背景情况:

单细胞质谱 (MS) 对于对代谢物和脂质进行敏感的多重分析至关重要.
单细胞脂质的结构阐明具有挑战性,特别是在高通量环境中,因为样本体积有限.
现有的方法在单细胞的综合结构分析和高通量要求方面扎.

研究的目的:

开发一个高通量单细胞MS平台,用于脂质分析和碳-碳双键 (C[双键,长度为m-dash]C) 位置同位素分辨率.
为了克服单细胞分析中小样本数量的局限性,用于详细的脂质结构确定.
提高单细胞脂质分析的准确性和深度,用于细胞表型和途径研究.

主要方法:

开发一个高通量单细胞MS平台.
在不和脂质中利用帕特诺-布希 (PB) 反应对C[双键,长度为m-dash]C激活.
用于结构阐明和C[双键,长度为m-dash]C位置异构体分析的协同MS.

主要成果:

在单细胞分析中,每分钟达到51个细胞的吞吐量.
在子类层面上结构性地表征了145种脂质.
确定了17种异构体脂质的相对丰度,它们在C[双键,长度为m-dash]C位置上有所不同,与标准MS1分析相比,其量化准确性得到了改进.

结论:

开发的平台可以进行高通量单细胞结构性脂管学分析.
量化脂质C[双键,长度为m-dash]C位置异构体显著提高了量化准确性.
该方法在大型单细胞队伍中对结构性脂质代谢网络进行深入分析是有效的,正如142个人类乳腺癌细胞所示.

更多相关视频

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Published on: October 25, 2019

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Tandem Mass Spectrometry

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...