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通过QuEChERS和高性能液态染色学分析,对样品制备程序进行比较研究,以确定阿雅华斯卡饮料中的主要化合物

Joana Gonçalves^1,2, Tiago Rosado^1,2, Mário Barroso³

¹Centro de Investigação em Ciências da Saúde (CICS-UBI), Universidade da Beira Interior, Covilhã, Portugal.

Phytochemical analysis : PCA

|May 3, 2024

概括

这项研究优化了QuEChERS提取方法,用于分析饮料中的DMT和β-卡博林等关键阿雅华斯卡化合物. 经过验证的分析方法为雅华斯卡样本分析提供了准确和精确的结果.

科学领域:

分析化学分析化学
药理学是指药理学,即药理学是指药理学.
法医科学法医科学法医科学

背景情况:

亚马逊传统的精神活性饮料阿雅瓦斯卡 (Ayahuasca) 面临着不受管制的消费,通常是在宗教环境中.
由于其精神活性特性和文化意义,了解其化学成分至关重要.

研究的目的:

为了比较三个小型化提取技术:分散液-液微提取 (DLLME),微提取通过包装吸附剂 (MEPS) 和QuEChERS.
选择和优化最有效的技术,同时提取主要的阿雅华斯卡化合物.

主要方法:

评估了DLLME,MEPS和QuEChERS用于提取N,N-二甲基三胺 (DMT) 和β-碳醇蛋白 (THH,harmine,harmaline,harmol,harmalol).
优化了QuEChERS参数,包括溶剂体积,吸附剂质量和旋转时间.
使用二极管阵列探测器 (HPLC-DAD) 的高性能液态染色学分析了提取物.

主要成果:

通过500μL的提取溶剂,85毫克的PSA吸附剂和4秒的旋转,优化了QuEChERS方法.
经过验证的方法证明了DMT (0.0161μg/mL) 和β-卡博林 (0.1610μg/mL) 的线性.
提取效率从60.2%到88.0%不等,检测和量化的极限较低.

结论:

优化的QuEChERS方法准确,精确和线性,用于分析亚亚瓦斯卡化合物.
这种经过验证的分析方法适用于对阿雅华斯卡饮料的定量分析.

关键词:

DLLME DLLME 在线播放美国的MEPS.奎瑟人奎瑟人亚亚瓦斯卡 (Ayahuasca) 是一种

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