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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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相关实验视频

Updated: Jun 27, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA
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基于Cas9代码密钥系统的多重RNA快速现场成像

Ruiwei Hu1,2, Wei Yang3, Jia Li1

  • 1The Center for Clinical Molecular Medical Detection, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400042, China.

Small methods
|May 3, 2024
PubMed
概括

一种新的Cas9代码密钥系统简化了RNA的现场成像,使得瘤细胞中的多mRNA能够快速,高效地检测. 这一突破提供了增强的临床瘤类型和分子调查能力.

关键词:
这就是CRISPR/Cas9的作用.FFPE的样本是 FFPE 的样本.关键探头编码器编码器在现场成像多重RNA.瘤类型的确定.

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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相关实验视频

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Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
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科学领域:

  • 分子生物学分子生物学
  • 生物技术是生物技术.
  • 基因组学就是基因组学.

背景情况:

  • 现有的RNA in situ成像方法通常涉及复杂的核酸自组装,导致繁的程序和效率的限制.
  • 对于生物样本中多重RNA分析的简化和更有效的技术的需求至关重要.

研究的目的:

  • 开发一种简化且高效的多重RNA in situ成像系统.
  • 在RNA检测中克服传统并行信号分析的局限性.

主要方法:

  • 开发一个使用关键探针 (KP) 编码器和CRISPR/Cas9信号输出器的Cas9代码密钥系统.
  • 整合Cas9代码密钥系统与T-链位移放大 (T-SDA) 进行信号放大.
  • 在瘤细胞和临床病理切片中应用多mRNA成像系统.

主要成果:

  • Cas9代码密钥系统使单个sgRNA/Cas9复合体实现高效的"多对一"并联信号传输.
  • 该系统展示了非附带分离活动依赖的自动信号输出.
  • 用单个步骤在不到一个小时内生成了快速的多mRNA成像配置文件,显示了高效率和简化反应过程.

结论:

  • 开发的Cas9代码密钥系统显著简化了RNA在位成像过程.
  • 这种方法提供了一种快速有效的多重RNA检测方法,对于临床应用至关重要.
  • 该系统为临床瘤类型和分子机制研究提供可靠的技术支持.