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非特异性过氧酶 (UPO) 可以通过控制反应pH值来调整氧化或化活性

Verity Barber¹, Tamara Mielke¹, Jared Cartwright²

¹Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK.

Chemistry (Weinheim an der Bergstrasse, Germany)

|May 3, 2024

概括

非特异性过氧酶 (UPO) 是多功能生物催化剂. 通过调整pH值,可以调整UPO进行选择性氧化或化反应,从而在生物催化中提供新的控制水平.

科学领域:

生物催化剂是一种生物催化剂.
酶学是一种酶学.
有机化学有机化学

背景情况:

非特异性过氧酶 (UPO) 是稳定,活跃的酶,利用过氧化进行选择性氧化.
与氧化酶 (CPO) 的结构相似性使UPO能够通过 hypohalous 酸生成催化化.

研究的目的:

为了研究非特异性 Peroxygenases 的pH 取决的催化模式.
为了证明UPO在不同的化学转化过程中的可调性.

主要方法:

利用像提摩尔这样的简单芳香化合物来测试不同pH值 (3.0和6.0) 的UPO活性.
进行准备性规模反应 (100毫克) 来分离产品.
设计了一种单级联反应,并连续调整pH值.

主要成果:

在pH值为3.0的情况下,UPO可以选择性地从醇中生产化产品,产量为60-72%.
在pH 6.0下,UPO可以选择性地产生氧化产品.
在4-乙二醇的单级联反应中,pH值从3.0转变为6.0,产生了连续的化和氧化产物,其合并转化率为82%.

结论:

UPOs具有可调节pH的催化活性,使得不同的氧化和化反应成为可能.
这种依赖pH值的鱼性使UPO成为各种化学合成的高度适应性的生物催化剂.
展示了一种新的一级联反应,用于使用UPO进行序列功能化.

关键词:

生物催化剂是一种生物催化剂.氧化过程中的氧化.过氧基因酶的使用.在此之前,UPO UPO UPO.和化和化.

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