中等链长的多氧甲酸盐由工程 Pseudomonas gessardii 使用乙酸盐作为碳来源生产
Woo Young Kim1, Seung-Jin Kim1, Hye-Rin Seo2
1Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Republic of Korea.
Journal of microbiology (Seoul, Korea)
|May 3, 2024
概括
Pseudomonas gessardii被设计为使用酸盐和酸盐生产中长链聚酸酸盐 (mcl-PHA). 这种增强的微生物菌株显示出从简单的碳来源可持续生产生物聚合物的潜力.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
背景情况:
- 从韩国分离出来的 Pseudomonas gessardii NIBRBAC000509957 在自然界产生多基酸 (PHAs).
- 这种菌株有效地利用乙酸盐和甲酸盐作为碳来源,以5g/L乙酸盐的最佳生长率.
- 代谢工程可以增强特定PHA类型的产生,例如中长链PHA (mcl-PHA).
研究的目的:
- 使用Pseudomonas gessardii优化mcl-PHA的产生.
- 调查乙酸盐和甲酸盐作为碳来源的利用,以促进微生物生长和PHA合成.
- 通过代谢工程来提高菌株的mcl-PHA产量.
主要方法:
- 鉴别和鉴定Pseudomonas gessardii NIBRBAC000509957.7. 的特征
- 对乙酸和甲酸用于细菌生长的评估.
- 过度表达酸盐和酸盐同化路径酶.
- 代谢工程策略,以提高mcl-PHA的生产.
- 用于mcl-PHA产量评估的料批发发酵.
主要成果:
- 观测到 Pseudomonas gessardii 在酸盐 5 g/L 的最佳生长.
- 过度表达同化通路酶显著增加了生长速度.
- 工程菌株产生了0.40g/L的mcl-PHA.
- 在料批发发酵中达到30.43%mcl-PHA的生物质含量.
结论:
- Pseudomonas gessardii可以通过代谢工程进行高效的mcl-PHA生产.
- 乙酸盐和甲酸盐是可行的碳来源,可以增强微生物生长和生物聚合物合成.
- 工程菌株具有从简单碳来源可持续生产有价值的mcl-PHA的潜力.
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